Title:
ニトロレダクターゼ酵素活性の測定法及び試薬
Document Type and Number:
Japanese Patent JP4838239
Kind Code:
B2
Abstract:
Disclosed are nitro-substituted squaraine reporter dyes and methods using such dyes for detecting nitroreductase enzyme activity and nitroreductase gene expression in cellular assays. The dyes are of the structure: in which Z1 and Z2 independently represent a phenyl or a naphthyl ring system; X and Y are selected from oxygen, sulphur, —CH═CH— and the group: R1 and R2 are selected from C1-C4 alkyl, —(CH2)n—P, —{(CH2)2—O}p—R6 and group W; where P is selected from COOR7, SO3− and OH, W is mono- or di-substituted nitrobenzyl, R6 is methyl or ethyl, R7 is selected from H, C1-C4 alkyl and CH2OC(O)R8, where R8 is methyl, or t-butyl, n is an integer from 1 to 10, and p is an integer from 1 to 3; R3 and R4 are selected from hydrogen, NO2, halogen, SO3−, C1-C4 alkoxy and —(CH2)m—COOR7; where R7 is hereinbefore defined and m is 0 or an integer from 1 to 5; R5 is C1-C6 alkyl optionally substituted with COOR7, SO3−, or OH; where R7 is hereinbefore defined; and at least one of groups R1, R2, R3 and R4 comprises at least one NO2 group. Also provided are methods for screening for a test agent whose effect upon nitroreductase enzyme activity and nitroreductase gene expression is to be determined.
More Like This:
WO/2020/036904 | NOVEL UREA-OXAZIRIDINES |
WO/2008/013831 | METHOD FOR PREPARING AN OXYGEN REDUCED FILM AND PACKAGE |
JP5393771 | Microbial detection and counting |
Inventors:
West, Richard Martin
Ismail, Rahman
Ismail, Rahman
Application Number:
JP2007514089A
Publication Date:
December 14, 2011
Filing Date:
May 24, 2005
Export Citation:
Assignee:
GE Healthcare UK Limited
International Classes:
C12Q1/26; C09B23/01; C09B23/02; C09B57/00; C09K11/06; C12Q1/32; G01N21/78
Domestic Patent References:
JP2003522333A | 2003-07-22 | |||
JP2000508709A | 2000-07-11 | |||
JPH07160028A | 1995-06-23 |
Foreign References:
WO2003087052A2 | 2003-10-23 |
Attorney, Agent or Firm:
Arakawa Satoshi
Hirokazu Ogura
Toshihisa Kurokawa
Hirokazu Ogura
Toshihisa Kurokawa