Title:
細菌性細胞溶解素の精製方法
Document Type and Number:
Japanese Patent JP4934591
Kind Code:
B2
Abstract:
The present application relates to a process for purification of a bacterial cytolysin comprising the steps of a) growing a culture of cells expressing bacterial cytolysin, b) mechanically breaking the culture of cells to form an extract, c) prefiltering the extract, d) binding soluble aggregated bacterial cytolysin contained in the extract in the presence of detergent to a hydrophobic interaction chromatography material under high salt (preferably 0.6-2M salt) conditions, and e) eluting bacterial cytolysin in the presence of detergent under low salt (preferably 0-0.2M salt) conditions.
Inventors:
Beams, Ralph, Leon
Goraz, Karine
Emmanuel, Martens
Van der Caman, Anne
Goraz, Karine
Emmanuel, Martens
Van der Caman, Anne
Application Number:
JP2007531717A
Publication Date:
May 16, 2012
Filing Date:
September 20, 2005
Export Citation:
Assignee:
GlaxoSmithKline Biologicals Society Anonymous
International Classes:
C07K14/315; A61K39/09; C07K1/113; C07K1/20; C12P21/02; A61K39/00; A61K39/07; A61K39/102; A61K39/116; A61P31/04; C12R1/46
Domestic Patent References:
| JP2006520205A | 2006-09-07 | |||
| JP2004359624A | 2004-12-24 |
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Eiichi Arai
Ishii Teiji
Satoshi Fujita
Eiichi Arai