Title:
組換えタンパク質のタンパク質分解性切断および精製のためのプロセス
Document Type and Number:
Japanese Patent JP4960701
Kind Code:
B2
Abstract:
The present invention relates to improved methods for protein purification of high-value heterologous proteins by providing fusion proteins suitable for affinity purification and improved and economical methods of proteolytic cleavage of fusion proteins. The methods are useful for large-scale production of purified recombinant proteins from plants, plant-derived tissue or plant cells. The invention aims to reduce the cost and improve the quality of downstream processing of heterologous proteins produced in plants and other biological production systems.
Inventors:
Mantilla, eina
Oliva, Björn Rals
Oliva, Björn Rals
Application Number:
JP2006524529A
Publication Date:
June 27, 2012
Filing Date:
August 27, 2004
Export Citation:
Assignee:
ORF, Lifter, Ekunie, H.F.
International Classes:
C07K1/22; C12N9/64; C12N15/62; C12N15/82; C12P21/06; A01H1/00; A01H5/00; C07K14/195; C07K19/00; C12N5/10; C12N9/50; C12N15/09
Foreign References:
| WO2000077174A1 | ||||
| US6048715 |
Other References:
Biochemistry,2001年,vol. 40,6240-6247
Attorney, Agent or Firm:
Shinjiro Ono
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Hiroko Ejiri
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Hiroko Ejiri