Title:
生残菌数推定方法及び保証菌数の設定方法
Document Type and Number:
Japanese Patent JP5409617
Kind Code:
B2
Abstract:
The present invention provides a method for estimating survival cell count, which is able to calculate an accurately estimated result of the survival cell count of a specific strain in a probiotic product with respect to its storage period, and to shorten the time for developing the product, and a method for setting a guaranteed cell count, which is able to readily calculate a guaranteed cell count of a specific strain in a probiotic product within its quality guarantee period. The method for estimating the survival cell count is characterized by estimating the survival cell count n t (CFU/g) of a specific strain contained in a composition after storage, in accordance with the following equation (I). (I) Log 10 n t = Log 10 n 0 - t × EXP{(A T × T + B T )w + (C T × T + D T )}, provided that t stands for the storage period (days) × 1/30, n t stands for the survival cell count (CFU/g) of the strain contained in the composition after the storage period t (day), n 0 stands for the viable cell count (CFU/g) of the strain contained in the composition at the initiation of storage, T stands for the storage temperature (°C), w stands for the water activity value of the composition, A T and C T stand for experimentally determined coefficients specific to the strain, and B T and D T stand for experimentally determined constants specific to the strain.
Inventors:
Fumiaki Abe
Ayako Uchijima
Horikome Ayako
Ayako Uchijima
Horikome Ayako
Application Number:
JP2010514558A
Publication Date:
February 05, 2014
Filing Date:
May 29, 2009
Export Citation:
Assignee:
Morinaga Milk Industry Co., Ltd.
International Classes:
C12Q1/06; G16Z99/00
Other References:
JPN6009029278; ＫＩＭ Ｓ．Ｓ．， ｅｔ ａｌ．: 'Ｓｕｒｖｉｖａｌ ｏｆ Ｌａｃｔｉｃ ａｃｉｄ Ｂａｃｔｅｒｉａ ｄｕｒｉｎｇ Ｍｉｃｒｏｗａｖｅ Ｖａｃｕｕｍ-ｄｒｙｉｎｇ ｏｆ Ｐｌａｉｎ Ｙｏｇｈｕｒｔ．' Ｌｅｂｅｎｓｍ．-Ｗｉｓｓ． ｕ-Ｔｅｃｈｎｏｌ． Ｖｏｌ．３０，Ｎｏ．６, 1977, ｐ．５７３-５７７
JPN6009029279; ＷＩＪＴＺＥＳ Ｔ．， ｅｔ ａｌ．: 'Ｄｅｖｅｌｏｐｍｅｎｔ ａｎｄ ｖａｌｉｄａｔｉｏｎ ｏｆ ａ ｃｏｍｂｉｎｅｄ ｔｅｍｐｅｒａｔｕｒｅ， ｗａｔｅｒ ａｃｔｉｖｉｔｙ， ｐＨ ｍｏｄｅｌ ｆｏｒ ｂａｃｔｅｒｉａｌ ｇｒｏｗｔｈ' Ｉｎｔ．Ｊ．Ｆｏｏｄ Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ．６３, 2001, ｐ．５７-６４
JPN6009029281; ＷＨＩＴＩＮＧ Ｒ．Ｃ．: 'Ｍｉｃｒｏｂｉａｌ Ｄａｔａｂａｓｅ Ｂｕｉｌｄｉｎｇ： Ｗｈａｔ Ｈａｖｅ Ｗｅ Ｌｅａｒｎｅｄ？' Ｆｏｏｄ Ｔｅｃｈｎｏｌ． Ｖｏｌ．５１，Ｎｏ．４, 1997, ｐ．８２-８４，８６
JPN6009029277; ＧＥＥＲＡＥＲＤ Ａ．Ｈ．， ｅｔ ａｌ．: 'Ｄｅｖｅｌｏｐｍｅｎｔ ｏｆ ａ ｎｏｖｅｌ ａｐｐｒｏａｃｈ ｆｏｒ ｓｅｃｏｎｄａｒｙ ｍｏｄｅｌｌｉｎｇ ｉｎ ｐｒｅｄｉｃｔｉｖｅ ｍｉｃｒｏｂｉｏｌｏｇｙ： ｉｎｃｏｒｐｏｒａｔｉｏｎ ｏｆ' Ｉｎｔ．Ｊ．Ｆｏｏｄ Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ．９１，Ｎｏ．３, 2004, ｐ．２２９-２４４
JPN6009029278; ＫＩＭ Ｓ．Ｓ．， ｅｔ ａｌ．: 'Ｓｕｒｖｉｖａｌ ｏｆ Ｌａｃｔｉｃ ａｃｉｄ Ｂａｃｔｅｒｉａ ｄｕｒｉｎｇ Ｍｉｃｒｏｗａｖｅ Ｖａｃｕｕｍ-ｄｒｙｉｎｇ ｏｆ Ｐｌａｉｎ Ｙｏｇｈｕｒｔ．' Ｌｅｂｅｎｓｍ．-Ｗｉｓｓ． ｕ-Ｔｅｃｈｎｏｌ． Ｖｏｌ．３０，Ｎｏ．６, 1977, ｐ．５７３-５７７
JPN6009029279; ＷＩＪＴＺＥＳ Ｔ．， ｅｔ ａｌ．: 'Ｄｅｖｅｌｏｐｍｅｎｔ ａｎｄ ｖａｌｉｄａｔｉｏｎ ｏｆ ａ ｃｏｍｂｉｎｅｄ ｔｅｍｐｅｒａｔｕｒｅ， ｗａｔｅｒ ａｃｔｉｖｉｔｙ， ｐＨ ｍｏｄｅｌ ｆｏｒ ｂａｃｔｅｒｉａｌ ｇｒｏｗｔｈ' Ｉｎｔ．Ｊ．Ｆｏｏｄ Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ．６３, 2001, ｐ．５７-６４
JPN6009029281; ＷＨＩＴＩＮＧ Ｒ．Ｃ．: 'Ｍｉｃｒｏｂｉａｌ Ｄａｔａｂａｓｅ Ｂｕｉｌｄｉｎｇ： Ｗｈａｔ Ｈａｖｅ Ｗｅ Ｌｅａｒｎｅｄ？' Ｆｏｏｄ Ｔｅｃｈｎｏｌ． Ｖｏｌ．５１，Ｎｏ．４, 1997, ｐ．８２-８４，８６
JPN6009029277; ＧＥＥＲＡＥＲＤ Ａ．Ｈ．， ｅｔ ａｌ．: 'Ｄｅｖｅｌｏｐｍｅｎｔ ｏｆ ａ ｎｏｖｅｌ ａｐｐｒｏａｃｈ ｆｏｒ ｓｅｃｏｎｄａｒｙ ｍｏｄｅｌｌｉｎｇ ｉｎ ｐｒｅｄｉｃｔｉｖｅ ｍｉｃｒｏｂｉｏｌｏｇｙ： ｉｎｃｏｒｐｏｒａｔｉｏｎ ｏｆ' Ｉｎｔ．Ｊ．Ｆｏｏｄ Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ．９１，Ｎｏ．３, 2004, ｐ．２２９-２４４
JPN6009029279; ＷＩＪＴＺＥＳ Ｔ．， ｅｔ ａｌ．: 'Ｄｅｖｅｌｏｐｍｅｎｔ ａｎｄ ｖａｌｉｄａｔｉｏｎ ｏｆ ａ ｃｏｍｂｉｎｅｄ ｔｅｍｐｅｒａｔｕｒｅ， ｗａｔｅｒ ａｃｔｉｖｉｔｙ， ｐＨ ｍｏｄｅｌ ｆｏｒ ｂａｃｔｅｒｉａｌ ｇｒｏｗｔｈ' Ｉｎｔ．Ｊ．Ｆｏｏｄ Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ．６３, 2001, ｐ．５７-６４
JPN6009029281; ＷＨＩＴＩＮＧ Ｒ．Ｃ．: 'Ｍｉｃｒｏｂｉａｌ Ｄａｔａｂａｓｅ Ｂｕｉｌｄｉｎｇ： Ｗｈａｔ Ｈａｖｅ Ｗｅ Ｌｅａｒｎｅｄ？' Ｆｏｏｄ Ｔｅｃｈｎｏｌ． Ｖｏｌ．５１，Ｎｏ．４, 1997, ｐ．８２-８４，８６
JPN6009029277; ＧＥＥＲＡＥＲＤ Ａ．Ｈ．， ｅｔ ａｌ．: 'Ｄｅｖｅｌｏｐｍｅｎｔ ｏｆ ａ ｎｏｖｅｌ ａｐｐｒｏａｃｈ ｆｏｒ ｓｅｃｏｎｄａｒｙ ｍｏｄｅｌｌｉｎｇ ｉｎ ｐｒｅｄｉｃｔｉｖｅ ｍｉｃｒｏｂｉｏｌｏｇｙ： ｉｎｃｏｒｐｏｒａｔｉｏｎ ｏｆ' Ｉｎｔ．Ｊ．Ｆｏｏｄ Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ．９１，Ｎｏ．３, 2004, ｐ．２２９-２４４
JPN6009029278; ＫＩＭ Ｓ．Ｓ．， ｅｔ ａｌ．: 'Ｓｕｒｖｉｖａｌ ｏｆ Ｌａｃｔｉｃ ａｃｉｄ Ｂａｃｔｅｒｉａ ｄｕｒｉｎｇ Ｍｉｃｒｏｗａｖｅ Ｖａｃｕｕｍ-ｄｒｙｉｎｇ ｏｆ Ｐｌａｉｎ Ｙｏｇｈｕｒｔ．' Ｌｅｂｅｎｓｍ．-Ｗｉｓｓ． ｕ-Ｔｅｃｈｎｏｌ． Ｖｏｌ．３０，Ｎｏ．６, 1977, ｐ．５７３-５７７
JPN6009029279; ＷＩＪＴＺＥＳ Ｔ．， ｅｔ ａｌ．: 'Ｄｅｖｅｌｏｐｍｅｎｔ ａｎｄ ｖａｌｉｄａｔｉｏｎ ｏｆ ａ ｃｏｍｂｉｎｅｄ ｔｅｍｐｅｒａｔｕｒｅ， ｗａｔｅｒ ａｃｔｉｖｉｔｙ， ｐＨ ｍｏｄｅｌ ｆｏｒ ｂａｃｔｅｒｉａｌ ｇｒｏｗｔｈ' Ｉｎｔ．Ｊ．Ｆｏｏｄ Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ．６３, 2001, ｐ．５７-６４
JPN6009029281; ＷＨＩＴＩＮＧ Ｒ．Ｃ．: 'Ｍｉｃｒｏｂｉａｌ Ｄａｔａｂａｓｅ Ｂｕｉｌｄｉｎｇ： Ｗｈａｔ Ｈａｖｅ Ｗｅ Ｌｅａｒｎｅｄ？' Ｆｏｏｄ Ｔｅｃｈｎｏｌ． Ｖｏｌ．５１，Ｎｏ．４, 1997, ｐ．８２-８４，８６
JPN6009029277; ＧＥＥＲＡＥＲＤ Ａ．Ｈ．， ｅｔ ａｌ．: 'Ｄｅｖｅｌｏｐｍｅｎｔ ｏｆ ａ ｎｏｖｅｌ ａｐｐｒｏａｃｈ ｆｏｒ ｓｅｃｏｎｄａｒｙ ｍｏｄｅｌｌｉｎｇ ｉｎ ｐｒｅｄｉｃｔｉｖｅ ｍｉｃｒｏｂｉｏｌｏｇｙ： ｉｎｃｏｒｐｏｒａｔｉｏｎ ｏｆ' Ｉｎｔ．Ｊ．Ｆｏｏｄ Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ．９１，Ｎｏ．３, 2004, ｐ．２２９-２４４
Attorney, Agent or Firm:
Masatake Shiga
Tadashi Takahashi
Takashi Watanabe
Suzuki Mitsuyoshi
Kazuya Nishi
Yasuhiko Murayama
Tadashi Takahashi
Takashi Watanabe
Suzuki Mitsuyoshi
Kazuya Nishi
Yasuhiko Murayama