Title:
リアルタイム核酸検出法と組成物
Document Type and Number:
Japanese Patent JP5518428
Kind Code:
B2
Abstract:
The present invention provides a composition of matter comprising: (a) a first part comprising at least one first nucleic acid primer that comprises (i) at least one first energy transfer element; and (ii) a nucleic acid sequence which is complementary to a nucleic acid sequence in at least a portion of a nucleic acid of interest; (b) a second part comprising at least one second nucleic acid primer that comprises: (i) at least one second energy transfer element; and (ii) a nucleic acid sequence which is identical to a nucleic acid sequence in at least a portion of said nucleic acid of interest; wherein said first nucleic acid primer does not comprise said second energy transfer element, and wherein said second nucleic acid primer does not comprise said first energy transfer element; wherein the first energy transfer element is capable of acting as an energy donor and the second energy transfer element is capable of acting as an energy acceptor, or the first energy transfer element is capable of acting as acting as an energy acceptor and the second energy transfer element is capable of acting as an energy donor; and wherein the sequences of the first nucleic acid primer and the second nucleic acid primer are selected such that if the nucleic acid of interest is present in the sample, said first and second nucleic acid primers will be incorporated into different complementary strands of a double stranded amplicon in sufficient proximity for energy transfer to occur between the first energy transfer element and the second energy transfer element of the incorporated primers; (c) a sample suspected of containing said nucleic acid of interest; and (d) reagents for carrying out nucleic acid strand extension.
Inventors:
Elaza Lavani
Janice Gee, Stavrianopoulos
James Jay, Donegan
Jack, Coleman
Dakai Liu
Janice Gee, Stavrianopoulos
James Jay, Donegan
Jack, Coleman
Dakai Liu
Application Number:
JP2009246566A
Publication Date:
June 11, 2014
Filing Date:
October 27, 2009
Export Citation:
Assignee:
Enzo Life Sciences, Inc.
International Classes:
C07K14/01; C12N15/09; C07K14/245; G01N33/53; C07K14/37; C07K14/415; C07K14/435; C07K16/00; C12M1/00; C12Q1/68; G01N37/00
Domestic Patent References:
| JP2001513623A | ||||
| JP9508525A | ||||
| JP2004004048A |
Foreign References:
| WO1999064432A1 | ||||
| WO1999028501A1 | ||||
| US6174670 |
Other References:
村松正明ほか編, 細部工学別冊ゲノムサイエンスシリーズ1 DNAマイクロアレイと最新PCR法-いま,ポストゲノムがおもしろい-, 株式会社秀潤社, 2000年3月, p.113-123
Anal. Biochem., vol. 292, pages 188-197 (2001)
Clin. Chem., vol. 43, pages 2262-2267 (1997)
Anal. Biochem., vol. 292, pages 188-197 (2001)
Clin. Chem., vol. 43, pages 2262-2267 (1997)
Attorney, Agent or Firm:
Hideto Asamura
Hajime Asamura
Naganuma Akio
Yukihiro Ikeda
Hajime Asamura
Naganuma Akio
Yukihiro Ikeda