Title:
ｍＲＮＡ結合プローブを用いる生存細胞の選択および単離
Document Type and Number:
Japanese Patent JP5753134
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To produce a cell line expressing at least one protein selected in advance.SOLUTION: The cell line provides a method for efficiently detecting mRNA and other RNA in a living cell in high reliability; and a method for specifying the cell in accordance with desired characters and separating the cells as necessary. The methods simplify conventional treatment processes significantly, shorten the time required for the practice of the same, and also provide new methods for research, such as, the selection of a cell producing a specific protein or an antisense oligonucleotide, the production of the cell line expressing the multiple number of proteins, the production of the knock out cell line for one or more proteins and the like.
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Inventors:
Kanbit Shekder
Gunter Brovel
Gunter Brovel
Application Number:
JP2012161649A
Publication Date:
July 22, 2015
Filing Date:
July 20, 2012
Export Citation:
Assignee:
Chromocell Corporation
International Classes:
C12N15/09; A01K67/027; C12N5/0735; C12N5/10; C12Q1/02; C12Q1/68
Domestic Patent References:
| JP2005511050A |
Foreign References:
| WO2002006524A1 |
Other References:
Proc. Natl. Acad. Sci. USA, (1998), 95, [20], p.11538-11543
J. Virol., (1997), 71, [12], p.9466-9474
BioTechniques, (1999), 26, [4], p.683-688
J. Biol. Chem., (2001), 276, [33], p.31305-31310
J. Virol., (1997), 71, [12], p.9466-9474
BioTechniques, (1999), 26, [4], p.683-688
J. Biol. Chem., (2001), 276, [33], p.31305-31310
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Natsuki Morishita