Title:
膵ホルモン産生細胞の製造法
Document Type and Number:
Japanese Patent JP5762979
Kind Code:
B2
Abstract:
The present invention provides a method of more efficiently producing pancreas cells, particularly pancreatic hormone-producing cells, a method of stably producing pancreas cells in a large amount by more efficiently inducing differentiation of stem cells into pancreas cells, a medicament containing a pancreas cells and a screening method using the cells. A method of producing pancreatic hormone-producing cells, including subjecting stem cells to the following steps (1) - (4):
(1) a step of cultivating stem cells in a medium containing an activator of activin receptor-like kinase-4,7 and a GSK3 inhibitor
(2) a step of cultivating the cells obtained in the aforementioned step (1) in a medium containing an activator of activin receptor-like kinase-4,7
(3) a step of cultivating the cells obtained in the aforementioned step (2) in a medium containing any one or more kinds selected from the group consisting of (a) retinoic acid receptor agonists, (b) at least one kind selected from the group consisting of inhibitors of AMP-activated protein kinase and/or activin receptor-like kinase-2,3,6, and BMP antagonists, and (c) inhibitors of activin receptor-like kinase-4,5,7
(4) a step of cultivating the cells obtained in the aforementioned stem (3).
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Inventors:
Masaki Hosoya
Yuya Kunisada
Masanobu Shoji
Noriko Yamazoe
Yuya Kunisada
Masanobu Shoji
Noriko Yamazoe
Application Number:
JP2011547740A
Publication Date:
August 12, 2015
Filing Date:
December 28, 2010
Export Citation:
Assignee:
Takeda GmbH
International Classes:
C12N5/0735; A61K35/39; C12N5/071; C12N5/10
Domestic Patent References:
| JP2002538779A | 2002-11-19 |
Foreign References:
| WO2009018453A1 | 2009-02-05 | |||
| WO2009012428A2 | 2009-01-22 | |||
| WO2009070592A2 | 2009-06-04 | |||
| WO2008033408A2 | 2008-03-20 |
Other References:
JPN6011018832; ＷＲＩＧＨＴＯＮ，Ｋ．Ｈ． ｅｔ ａｌ: 'Ｔｒａｎｓｆｏｒｍｉｎｇ Ｇｒｏｗｔｈ Ｆａｃｔｏｒ beta Ｃａｎ Ｓｔｉｍｕｌａｔｅ Ｓｍａｄ１ Ｐｈｏｓｐｈｏｒｙｌａｔｉｏｎ Ｉｎｄｅｐｅｎｄｅｎｔｌｙ ｏｆ Ｂｏｎｅ Ｍｏｒｐｈｏｇｅｎｉｃ' Ｊ Ｂｉｏｌ Ｃｈｅｍ Ｖｏｌ．２８４， Ｎｏ．１５, 20090410, ｐ．９７５５-９７６３
JPN6011018838; ＹＵ，Ｐ．Ｂ． ｅｔ ａｌ: 'Ｄｏｒｓｏｍｏｒｐｈｉｎ ｉｎｈｉｂｉｔｓ ＢＭＰ ｓｉｇｎａｌｓ ｒｅｑｕｉｒｅｄ ｆｏｒ ｅｍｂｒｙｏｇｅｎｅｓｉｓ ａｎｄ ｉｒｏｎ ｍｅｔａｂｏｌｉｓｍ' Ｎａｔ Ｃｈｅｍ Ｂｉｏｌ Ｖｏｌ．４， Ｎｏ．１, 2008, ｐ．３３-４１
JPN6011018838; ＹＵ，Ｐ．Ｂ． ｅｔ ａｌ: 'Ｄｏｒｓｏｍｏｒｐｈｉｎ ｉｎｈｉｂｉｔｓ ＢＭＰ ｓｉｇｎａｌｓ ｒｅｑｕｉｒｅｄ ｆｏｒ ｅｍｂｒｙｏｇｅｎｅｓｉｓ ａｎｄ ｉｒｏｎ ｍｅｔａｂｏｌｉｓｍ' Ｎａｔ Ｃｈｅｍ Ｂｉｏｌ Ｖｏｌ．４， Ｎｏ．１, 2008, ｐ．３３-４１
Attorney, Agent or Firm:
Takashima Ichi
Kyoko Doi
Mitsunori Kamata
Yaeko Tamura
Kenji Yamamoto
Murata Miyuki
Junzo Koike
Hirofumi Toma
Kyoko Doi
Mitsunori Kamata
Yaeko Tamura
Kenji Yamamoto
Murata Miyuki
Junzo Koike
Hirofumi Toma