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Title:
方法および装置
Document Type and Number:
Japanese Patent JP5766284
Kind Code:
B2
Abstract:
A method for amplifying nucleic acid from a higher eukaryotic, such as mammalian or plant, nucleic acid source, the method comprising (a) contacting a sampling device with the source of higher eukaryotic, such as mammalian or plant, nucleic acid such that following said contacting, higher eukaryotic such as mammalian or plant nucleic acid-containing material is adhered to at least part of the sampling device, wherein the sampling device, or part thereof to which the nucleic acid-containing material is adhered, is made of a suitable polymeric material; (b) introducing the sampling device or part thereof to which the nucleic acid-containing material is adhered into a reaction vessel which contains a reaction mixture for carrying out a nucleic acid amplification reaction, without any prior treatment of the nucleic acid-containing material; and (c) performing a nucleic acid amplification reaction.

Inventors:
Paul Gerald Devenham
David John Moore
Application Number:
JP2013514785A
Publication Date:
August 19, 2015
Filing Date:
June 17, 2011
Export Citation:
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Assignee:
LGC Limited
International Classes:
C12N15/09; C12M1/00; C12M1/26; C12Q1/68
Domestic Patent References:
JP2005110687A
Foreign References:
WO2005032377A1
US6103192
WO1999034214A1
WO2008030820A1
Other References:
INSTRUCTIONS SHEET OF PHUSION HUMAN SPECIMEN DIRECT PCR KIT (THERMO SCIENTIFIC),[ONLINE],2010年 6月14日,URL,http://www.finnzymes.com/pdf/phusion_human_specimen_direct_pcr_kit_datasheet_fl50_1_0_low.pdf
DATA OF PUBLICATION OF XP055012357 (PHUSION HUMAN SPECIMEN DIRECT PCR KIT),[ONLINE],2010年 6月14日,URL,http://
FANG-CHIN WU,GENETIC POLYMORPHISMS OF 17 Y-CHROMOSOMAL SHORT TANDEM REPEAT LOCI IN ATAYAL POPULATION OF TAIWAN,CROATIAN MEDICAL JOURNAL,2009年 6月 1日,V50 N3,P313-320
Attorney, Agent or Firm:
Yasuhiko Murayama
Masatake Shiga
Takashi Watanabe
Shinya Mitsuhiro