グルコース測定用バイオセンサ

Japanese Patent JP5792214

B2

An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.

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Takako Yada
Hiroshi Miyamoto
Honda Tsusai

JP2013031338A

October 07, 2015

February 20, 2013

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Ikeda Shokuken Co., Ltd.

C12Q1/32; C12M1/34; C12N15/09

JP2008154572A
JP2004329143A
JP2007289148A

WO2007139013A1
WO2008001903A1

Biochim. Biophys. Acta,1967年,Vol.139, No.2,pp.277-293
Biosci. Biotechnol. Biochem.,2006年 3月,Vol.70, No.3,pp.654-659

Seiji Ohno
Koji Morita
Reiko Tanaka
Yuko Matsuniya
Natsuki Ito
Keiko Kanemoto

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