Title:
バイオ人工臓器作製方法
Document Type and Number:
Japanese Patent JP5797113
Kind Code:
B2
Abstract:
Disclosed are: a method for inducing the differentiation of stem cells into tissue cells; a bioartificial organ which comprises the tissue cells; and a material for medical purposes, which comprises the bioartificial organ. Stem cells capable of proliferation, self-replication and differentiation are used and cultured by a hanging-drop method to three-dimensionally construct a cell mass of embryoid bodies, and the cell mass is cultured in the presence of HGF, GDNF, b-FGF, BMP7 and EGF. In this manner, the stem cells can be differentiated into tissue cells. A bioartificial organ can be produced using cells that have been differentiated into the tissue cells. Further, a material for medical purposes can be provided.
Inventors:
Shinji Kitamura
Hiroshi Makino
Hiroshi Makino
Application Number:
JP2011527652A
Publication Date:
October 21, 2015
Filing Date:
August 11, 2010
Export Citation:
Assignee:
Organ Technologies Co., Ltd.
International Classes:
A61L27/00; C12N5/0735; C12N5/071; C12N5/074
Domestic Patent References:
| JP2004275079A | 2004-10-07 | |||
| JP2004275079A | 2004-10-07 |
Foreign References:
| US20050074875A1 | 2005-04-07 | |||
| WO2008151254A1 | 2008-12-11 |
Other References:
JPN6010062066; 田中啓之: '胚性幹細胞（ＥＳ細胞）からの分化誘導' 腎臓 Ｖｏｌ．２８， Ｎｏ．３, 2006, ｐｐ．１７５-９
JPN6010062068; 前嶋明人: '７．尿細管再生に関する最近の知見' Ａｎｎｕａｌ Ｒｅｖｉｅｗ 腎臓 ２００９ , 20090130, ｐｐ．４３-９
JPN6010062072; 喜多村真治、槇野博史: '成体腎臓幹／前駆細胞からの腎臓構造再構築-培養条件の検討' 日本腎臓学会誌 Ｖｏｌ．５２， Ｎｏ．３, 201005, ｐｐ．３８５
JPN6014044222; ＰＥＰＩＣＥＬＬＩ，Ｃ． ｅｔ ａｌ．: 'ＧＤＮＦ ｉｎｄｕｃｅｓ ｂｒａｎｃｈｉｎｇ ａｎｄ ｉｎｃｒｅａｓｅｄ ｃｅｌｌ ｐｒｏｌｉｆｅｒａｔｉｏｎ ｉｎ ｔｈｅ ｕｒｅｔｅｒ ｏｆ ｔｈｅ ｍｏｕｓｅ．' Ｄｅｖ． Ｂｉｏｌ． Ｖｏｌ．１９２， Ｎｏ．１, 19971201, ｐｐ．１９３-８
JPN6014044224; ＫＩＭ，Ｄ． ＡＮＤ ＤＲＥＳＳＬＥＲ，Ｇ．Ｒ．: 'Ｎｅｐｈｒｏｇｅｎｉｃ ｆａｃｔｏｒｓ ｐｒｏｍｏｔｅ ｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ ｏｆ ｍｏｕｓｅ ｅｍｂｒｙｏｎｉｃ ｓｔｅｍ ｃｅｌｌｓ ｉｎｔｏ ｒｅｎａｌ ｅｐｉｔｈｅｌｉａ．' Ｊ． Ａｍ． Ｓｏｃ． Ｎｅｐｈｒｏｌ． Ｖｏｌ．１６， Ｎｏ．１２, 200512, ｐｐ．３５２７-３４
JPN6014044225; ＭＡＥＳＨＩＭＡ，Ａ． ｅｔ ａｌ．: 'Ａｄｕｌｔ ｋｉｄｎｅｙ ｔｕｂｕｌａｒ ｃｅｌｌ ｐｏｐｕｌａｔｉｏｎ ｓｈｏｗｉｎｇ ｐｈｅｎｏｔｙｐｉｃ ｐｌａｓｔｉｃｉｔｙ， ｔｕｂｕｌｏｇｅｎｉｃ ｃａｐａｃｉｔｙ， ａｎｄ ｉｎｔｅｇｒ' Ｊ． Ａｍ． Ｓｏｃ． Ｎｅｐｈｒｏｌ． Ｖｏｌ．１７， Ｎｏ．１, 200601, ｐｐ．１８８-９８
JPN6014044226; ＰＥＲＡＮＴＯＮＩ，Ａ．Ｏ． ｅｔ ａｌ．: 'Ｂａｓｉｃ ｆｉｂｒｏｂｌａｓｔ ｇｒｏｗｔｈ ｆａｃｔｏｒ ｃａｎ ｍｅｄｉａｔｅ ｔｈｅ ｅａｒｌｙ ｉｎｄｕｃｔｉｖｅ ｅｖｅｎｔｓ ｉｎ ｒｅｎａｌ ｄｅｖｅｌｏｐｍｅｎｔ．' Ｐｒｏｃ． Ｎａｔｌ． Ａｃａｄ． Ｓｃｉ． ＵＳＡ Ｖｏｌ．９２， Ｎｏ．１０, 19950509, ｐｐ．４６９６-７００
JPN6014044227; ＭＯＲＩＺＡＮＥ，Ｒ． ｅｔ ａｌ．: 'Ｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ ｏｆ ｍｕｒｉｎｅ ｅｍｂｒｙｏｎｉｃ ｓｔｅｍ ａｎｄ ｉｎｄｕｃｅｄ ｐｌｕｒｉｐｏｔｅｎｔ ｓｔｅｍ ｃｅｌｌｓ ｔｏ ｒｅｎａｌ ｌｉｎｅａｇｅ ｉｎ ｖｉｔｒ' Ｂｉｏｃｈｅｍ． Ｂｉｏｐｈｙｓ． Ｒｅｓ． Ｃｏｍｍｕｎ． Ｖｏｌ．３９０， Ｎｏ．４, 20091225, ｐｐ．１３３４-９
JPN6014044228; ＫＩＮＯＭＵＲＡ，Ｍ． ｅｔ ａｌ．: 'Ａｍｅｌｉｏｒａｔｉｏｎ ｏｆ ｃｉｓｐｌａｔｉｎ-ｉｎｄｕｃｅｄ ａｃｕｔｅ ｒｅｎａｌ ｉｎｊｕｒｙ ｂｙ ｒｅｎａｌ ｐｒｏｇｅｎｉｔｏｒ-ｌｉｋｅ ｃｅｌｌｓ ｄｅｒｉｖｅｄ ｆｒｏｍ ｔｈｅ' Ｃｅｌｌ Ｔｒａｎｓｐｌａｎｔ． Ｖｏｌ．１７， Ｎｏ．１-２, 2008, ｐｐ．１４３-５８
JPN6014044219; ＫＩＴＡＭＵＲＡ，Ｓ． ｅｔ ａｌ．: 'Ｅｓｔａｂｌｉｓｈｍｅｎｔ ａｎｄ ｃｈａｒａｃｔｅｒｉｚａｔｉｏｎ ｏｆ ｒｅｎａｌ ｐｒｏｇｅｎｉｔｏｒ ｌｉｋｅ ｃｅｌｌｓ ｆｒｏｍ Ｓ３ ｓｅｇｍｅｎｔ ｏｆ ｎｅｐｈｒｏｎ ｉｎ ｒａｔ' ＦＡＳＥＢ Ｊ． Ｖｏｌ．１９， Ｎｏ．１３, 200511, ｐｐ．１７８９-９７
JPN6014044221; ＰＥＹ，Ｒ． ｅｔ ａｌ．: 'Ａ ｎｅｗ ｉｎ ｖｉｔｒｏ ｂｉｏａｓｓａｙ ｆｏｒ ｃｙｓｔ ｆｏｒｍａｔｉｏｎ ｂｙ ｒｅｎａｌ ｃｅｌｌｓ ｆｒｏｍ ａｎ ａｕｔｏｓｏｍａｌ ｄｏｍｉｎａｎｔ ｒａｔ ｍｏｄｅｌ ｏｆ ｐｏ' Ｉｎ Ｖｉｔｒｏ Ｃｅｌｌ Ｄｅｖ． Ｂｉｏｌ． Ａｎｉｍ． Ｖｏｌ．３５， Ｎｏ．１０, 199911, ｐｐ．５７１-９
JPN6014044219; ＫＩＴＡＭＵＲＡ，Ｓ． ｅｔ ａｌ．: 'Ｅｓｔａｂｌｉｓｈｍｅｎｔ ａｎｄ ｃｈａｒａｃｔｅｒｉｚａｔｉｏｎ ｏｆ ｒｅｎａｌ ｐｒｏｇｅｎｉｔｏｒ ｌｉｋｅ ｃｅｌｌｓ ｆｒｏｍ Ｓ３ ｓｅｇｍｅｎｔ ｏｆ ｎｅｐｈｒｏｎ ｉｎ ｒａｔ' ＦＡＳＥＢ Ｊ． Ｖｏｌ．１９， Ｎｏ．１３, 200511, ｐｐ．１７８９-９７
JPN6014044221; ＰＥＹ，Ｒ． ｅｔ ａｌ．: 'Ａ ｎｅｗ ｉｎ ｖｉｔｒｏ ｂｉｏａｓｓａｙ ｆｏｒ ｃｙｓｔ ｆｏｒｍａｔｉｏｎ ｂｙ ｒｅｎａｌ ｃｅｌｌｓ ｆｒｏｍ ａｎ ａｕｔｏｓｏｍａｌ ｄｏｍｉｎａｎｔ ｒａｔ ｍｏｄｅｌ ｏｆ ｐｏ' Ｉｎ Ｖｉｔｒｏ Ｃｅｌｌ Ｄｅｖ． Ｂｉｏｌ． Ａｎｉｍ． Ｖｏｌ．３５， Ｎｏ．１０, 199911, ｐｐ．５７１-９
JPN6010062068; 前嶋明人: '７．尿細管再生に関する最近の知見' Ａｎｎｕａｌ Ｒｅｖｉｅｗ 腎臓 ２００９ , 20090130, ｐｐ．４３-９
JPN6010062072; 喜多村真治、槇野博史: '成体腎臓幹／前駆細胞からの腎臓構造再構築-培養条件の検討' 日本腎臓学会誌 Ｖｏｌ．５２， Ｎｏ．３, 201005, ｐｐ．３８５
JPN6014044222; ＰＥＰＩＣＥＬＬＩ，Ｃ． ｅｔ ａｌ．: 'ＧＤＮＦ ｉｎｄｕｃｅｓ ｂｒａｎｃｈｉｎｇ ａｎｄ ｉｎｃｒｅａｓｅｄ ｃｅｌｌ ｐｒｏｌｉｆｅｒａｔｉｏｎ ｉｎ ｔｈｅ ｕｒｅｔｅｒ ｏｆ ｔｈｅ ｍｏｕｓｅ．' Ｄｅｖ． Ｂｉｏｌ． Ｖｏｌ．１９２， Ｎｏ．１, 19971201, ｐｐ．１９３-８
JPN6014044224; ＫＩＭ，Ｄ． ＡＮＤ ＤＲＥＳＳＬＥＲ，Ｇ．Ｒ．: 'Ｎｅｐｈｒｏｇｅｎｉｃ ｆａｃｔｏｒｓ ｐｒｏｍｏｔｅ ｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ ｏｆ ｍｏｕｓｅ ｅｍｂｒｙｏｎｉｃ ｓｔｅｍ ｃｅｌｌｓ ｉｎｔｏ ｒｅｎａｌ ｅｐｉｔｈｅｌｉａ．' Ｊ． Ａｍ． Ｓｏｃ． Ｎｅｐｈｒｏｌ． Ｖｏｌ．１６， Ｎｏ．１２, 200512, ｐｐ．３５２７-３４
JPN6014044225; ＭＡＥＳＨＩＭＡ，Ａ． ｅｔ ａｌ．: 'Ａｄｕｌｔ ｋｉｄｎｅｙ ｔｕｂｕｌａｒ ｃｅｌｌ ｐｏｐｕｌａｔｉｏｎ ｓｈｏｗｉｎｇ ｐｈｅｎｏｔｙｐｉｃ ｐｌａｓｔｉｃｉｔｙ， ｔｕｂｕｌｏｇｅｎｉｃ ｃａｐａｃｉｔｙ， ａｎｄ ｉｎｔｅｇｒ' Ｊ． Ａｍ． Ｓｏｃ． Ｎｅｐｈｒｏｌ． Ｖｏｌ．１７， Ｎｏ．１, 200601, ｐｐ．１８８-９８
JPN6014044226; ＰＥＲＡＮＴＯＮＩ，Ａ．Ｏ． ｅｔ ａｌ．: 'Ｂａｓｉｃ ｆｉｂｒｏｂｌａｓｔ ｇｒｏｗｔｈ ｆａｃｔｏｒ ｃａｎ ｍｅｄｉａｔｅ ｔｈｅ ｅａｒｌｙ ｉｎｄｕｃｔｉｖｅ ｅｖｅｎｔｓ ｉｎ ｒｅｎａｌ ｄｅｖｅｌｏｐｍｅｎｔ．' Ｐｒｏｃ． Ｎａｔｌ． Ａｃａｄ． Ｓｃｉ． ＵＳＡ Ｖｏｌ．９２， Ｎｏ．１０, 19950509, ｐｐ．４６９６-７００
JPN6014044227; ＭＯＲＩＺＡＮＥ，Ｒ． ｅｔ ａｌ．: 'Ｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ ｏｆ ｍｕｒｉｎｅ ｅｍｂｒｙｏｎｉｃ ｓｔｅｍ ａｎｄ ｉｎｄｕｃｅｄ ｐｌｕｒｉｐｏｔｅｎｔ ｓｔｅｍ ｃｅｌｌｓ ｔｏ ｒｅｎａｌ ｌｉｎｅａｇｅ ｉｎ ｖｉｔｒ' Ｂｉｏｃｈｅｍ． Ｂｉｏｐｈｙｓ． Ｒｅｓ． Ｃｏｍｍｕｎ． Ｖｏｌ．３９０， Ｎｏ．４, 20091225, ｐｐ．１３３４-９
JPN6014044228; ＫＩＮＯＭＵＲＡ，Ｍ． ｅｔ ａｌ．: 'Ａｍｅｌｉｏｒａｔｉｏｎ ｏｆ ｃｉｓｐｌａｔｉｎ-ｉｎｄｕｃｅｄ ａｃｕｔｅ ｒｅｎａｌ ｉｎｊｕｒｙ ｂｙ ｒｅｎａｌ ｐｒｏｇｅｎｉｔｏｒ-ｌｉｋｅ ｃｅｌｌｓ ｄｅｒｉｖｅｄ ｆｒｏｍ ｔｈｅ' Ｃｅｌｌ Ｔｒａｎｓｐｌａｎｔ． Ｖｏｌ．１７， Ｎｏ．１-２, 2008, ｐｐ．１４３-５８
JPN6014044219; ＫＩＴＡＭＵＲＡ，Ｓ． ｅｔ ａｌ．: 'Ｅｓｔａｂｌｉｓｈｍｅｎｔ ａｎｄ ｃｈａｒａｃｔｅｒｉｚａｔｉｏｎ ｏｆ ｒｅｎａｌ ｐｒｏｇｅｎｉｔｏｒ ｌｉｋｅ ｃｅｌｌｓ ｆｒｏｍ Ｓ３ ｓｅｇｍｅｎｔ ｏｆ ｎｅｐｈｒｏｎ ｉｎ ｒａｔ' ＦＡＳＥＢ Ｊ． Ｖｏｌ．１９， Ｎｏ．１３, 200511, ｐｐ．１７８９-９７
JPN6014044221; ＰＥＹ，Ｒ． ｅｔ ａｌ．: 'Ａ ｎｅｗ ｉｎ ｖｉｔｒｏ ｂｉｏａｓｓａｙ ｆｏｒ ｃｙｓｔ ｆｏｒｍａｔｉｏｎ ｂｙ ｒｅｎａｌ ｃｅｌｌｓ ｆｒｏｍ ａｎ ａｕｔｏｓｏｍａｌ ｄｏｍｉｎａｎｔ ｒａｔ ｍｏｄｅｌ ｏｆ ｐｏ' Ｉｎ Ｖｉｔｒｏ Ｃｅｌｌ Ｄｅｖ． Ｂｉｏｌ． Ａｎｉｍ． Ｖｏｌ．３５， Ｎｏ．１０, 199911, ｐｐ．５７１-９
JPN6014044219; ＫＩＴＡＭＵＲＡ，Ｓ． ｅｔ ａｌ．: 'Ｅｓｔａｂｌｉｓｈｍｅｎｔ ａｎｄ ｃｈａｒａｃｔｅｒｉｚａｔｉｏｎ ｏｆ ｒｅｎａｌ ｐｒｏｇｅｎｉｔｏｒ ｌｉｋｅ ｃｅｌｌｓ ｆｒｏｍ Ｓ３ ｓｅｇｍｅｎｔ ｏｆ ｎｅｐｈｒｏｎ ｉｎ ｒａｔ' ＦＡＳＥＢ Ｊ． Ｖｏｌ．１９， Ｎｏ．１３, 200511, ｐｐ．１７８９-９７
JPN6014044221; ＰＥＹ，Ｒ． ｅｔ ａｌ．: 'Ａ ｎｅｗ ｉｎ ｖｉｔｒｏ ｂｉｏａｓｓａｙ ｆｏｒ ｃｙｓｔ ｆｏｒｍａｔｉｏｎ ｂｙ ｒｅｎａｌ ｃｅｌｌｓ ｆｒｏｍ ａｎ ａｕｔｏｓｏｍａｌ ｄｏｍｉｎａｎｔ ｒａｔ ｍｏｄｅｌ ｏｆ ｐｏ' Ｉｎ Ｖｉｔｒｏ Ｃｅｌｌ Ｄｅｖ． Ｂｉｏｌ． Ａｎｉｍ． Ｖｏｌ．３５， Ｎｏ．１０, 199911, ｐｐ．５７１-９
Attorney, Agent or Firm:
Masahiro Nanjo
Takashi Matsuzaki
Takashi Matsuzaki