Title:
アルカリ性フィード
Document Type and Number:
Japanese Patent JP5798193
Kind Code:
B2
Abstract:
A method for cultivating a bacterial cell comprising the addition of an amino acid in an alkaline solution used for pH regulation. Also an aspect is a method for producing a polypeptide comprising the steps of a) providing a bacterial cell comprising a nucleic acid encoding the polypeptide, b) cultivating the provided cell, c) adjusting the pH value during the cultivating with a basic solution comprising an amino acid, d) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide.
Inventors:
Shunts, christian
Application Number:
JP2013526412A
Publication Date:
October 21, 2015
Filing Date:
August 25, 2011
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12P21/02; C12N1/21; C12N15/09
Domestic Patent References:
| JP2000501936A |
Foreign References:
| WO2003048374A1 |
Attorney, Agent or Firm:
Patent business corporation Tsukuni