Title:
連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法
Document Type and Number:
Japanese Patent JP5808742
Kind Code:
B2
Abstract:
Described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d -1 , and a cell density of less than about 2X10 7 cell/mL. Also described herein is a method for producing a polypeptide and/or virus of interest in a continuous cell culture, the method comprising culturing mammalian cells expressing the polypeptide and/or virus of interest in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d -1 , and a cell density of less than about 2X10 7 cell/mL; and recovering the polypeptide and/or virus of interest from medium of the cell culture system.
Inventors:
Grill Berger, Leopold
Lighter, manfred
Fly chandellu, daniel
Lighter, manfred
Fly chandellu, daniel
Application Number:
JP2012522190A
Publication Date:
November 10, 2015
Filing Date:
August 02, 2010
Export Citation:
Assignee:
Baxter Healthcare SA
BAXTER INTERNATIONAL INCORP0RATED
BAXTER INTERNATIONAL INCORP0RATED
International Classes:
C12P21/02; C12M3/00; C12N5/071; C12N5/10; C12N7/00; C12N9/48
Domestic Patent References:
| JP2009521941A |
Foreign References:
| WO2009086309A2 |
Other References:
J. Biotechnol.,1992年,vol.22, no.1-2,pp.41-50
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita