Title:
一本鎖オリゴヌクレオチド変異ベクター
Document Type and Number:
Japanese Patent JP5813571
Kind Code:
B2
Abstract:
The invention concerns the introduction of predetermined genetic changes in target genes of a living cell by introducing an oligodeoxynucleotide encoding the predetermined change. The oligodeoxynucleotides are effective in animal, plant and bacterial cells. Specific end modifications that greatly increase the effectiveness of the oligodeoxynucleotides in bacteria are described. Surprisingly, unmodified oligodeoxynucleotides can be as effective in mammalian cells, including in vivo hepatocytes, as the modified nucleotides and can be as effective or more effective than chimeric oligonucleotides that consist of a mixture of deoxynucleotides and 2'-O-methyl ribonucleotides.
Inventors:
Mets, Richard, A.
Frank, Bruce, Elle.
Walter, Debra, M.
Frank, Bruce, Elle.
Walter, Debra, M.
Application Number:
JP2012108528A
Publication Date:
November 17, 2015
Filing Date:
May 10, 2012
Export Citation:
Assignee:
Chives International, L.P.
International Classes:
A01K67/027; A61K31/7125; A61K9/127; A61K47/34; A61K47/48; A61K48/00; A61P1/16; A61P43/00; C12N5/10; C12N15/05; C12N15/09; C12N15/10; C12Q1/02
Domestic Patent References:
| JP9506511A | ||||
| JP63269985A | ||||
| JP5222088A |
Foreign References:
| WO1998049350A1 | ||||
| WO1999007865A1 | ||||
| WO1998058942A1 |
Other References:
Proc. Natl. Acad. Sci. USA,米国,1988年 1月,Vol.85,No.2,P.524-528
New Biologist,米国,1989年11月,Vol.1,No.2,P.223-227
Mol. Cell. Biochem.,1990年 2月,Vol.92,No.2,P.107-116
Proc. Natl. Acad. Sci. USA,米国,1986年10月 1日,Vol.83,No.19,P.7177-7181
今堀・山川監修,生化学辞典,日本,(株)東京化学同人,1998年10月 8日,第3版,第1刷,P.304,1253,1317
Bertling,K.H.,et al.,“Short DNA fragments induce site specific recombination in mammalian cells”,Mol. Cell. Biochem.,1990年 2月,Vol.92,No.2,P.107-116
New Biologist,米国,1989年11月,Vol.1,No.2,P.223-227
Mol. Cell. Biochem.,1990年 2月,Vol.92,No.2,P.107-116
Proc. Natl. Acad. Sci. USA,米国,1986年10月 1日,Vol.83,No.19,P.7177-7181
今堀・山川監修,生化学辞典,日本,(株)東京化学同人,1998年10月 8日,第3版,第1刷,P.304,1253,1317
Bertling,K.H.,et al.,“Short DNA fragments induce site specific recombination in mammalian cells”,Mol. Cell. Biochem.,1990年 2月,Vol.92,No.2,P.107-116
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Eiichi Arai
Natsuo Tanaka
Kosemura Akiko
Satoshi Fujita
Eiichi Arai
Natsuo Tanaka
Kosemura Akiko