Title:
免疫グロブリン溶液を精製するための方法
Document Type and Number:
Japanese Patent JP5814951
Kind Code:
B2
Abstract:
Herein is reported a method for purifying cell cultivation supernatants either directly after fermentation or after one or more preliminary purification steps, such as protein A affinity chromatography. By adjusting the pH value in the acid range and subsequent incubation of the acidified solution host cell nucleic acid and host cell protein can be precipitated but the target polypeptide remains in solution. Thereafter the precipitate and therewith the contaminating host cell components can be removed by a simple physical separation step.
Inventors:
Binder Binzenz
Akemeier christian
Stewarts ferritus
Akemeier christian
Stewarts ferritus
Application Number:
JP2012556507A
Publication Date:
November 17, 2015
Filing Date:
March 09, 2011
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C07K1/30; C07K16/00
Foreign References:
| WO2004024752A1 |
Other References:
Annals of the New York Academy of Science,1994年,Vol.745,p.222-231
Attorney, Agent or Firm:
Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto