Title:
抗体を得るための方法
Document Type and Number:
Japanese Patent JP5820823
Kind Code:
B2
Abstract:
The present disclosure relates to a method for the manufacture of recombinant antibody molecules comprising culturing a host cell sample transformed with an expression vector encoding a recombinant antibody molecule; adding an extraction buffer to the sample; and subjecting the sample to a heat treatment step; wherein the pH of the sample is detected after addition of the extraction buffer, and optionally adjusted, to ensure that the pH of the sample is 6 to 9 prior to the heat treatment step.
Inventors:
Birgisher, Jean-Pascal Pierre
Basset, Philip Jonathan
Pearce-Higgins, Mark Robert
Kenny, Andrew John
Basset, Philip Jonathan
Pearce-Higgins, Mark Robert
Kenny, Andrew John
Application Number:
JP2012551605A
Publication Date:
November 24, 2015
Filing Date:
February 02, 2011
Export Citation:
Assignee:
Yusebe Pharma Societe Anonym
International Classes:
C12P21/08; C07K16/00; C12N15/09
Domestic Patent References:
| JP2008520225A | ||||
| JP2007502609A |
Foreign References:
| US5665866 |
Attorney, Agent or Firm:
Asamura patent office
Hideto Asamura
Hajime Asamura
Shinichi Inoue
Yukihiro Ikeda
Hideto Asamura
Hajime Asamura
Shinichi Inoue
Yukihiro Ikeda