Title:
試料の構造を空間的に高分解能で結像するための装置および方法
Document Type and Number:
Japanese Patent JP5826494
Kind Code:
B2
Abstract:
Apparatus and method for high spatial resolution imaging of a sample's structure, including a diffraction-limited resolution volume with a plurality of dye molecules which can be switched between different states and have a distribution density which is greater than the inverse of the diffraction-limited resolution volume, where at least one state is fluorescing, the fluorescence being collected by an objective lens and imaged on a spatially resolving detector by an optical system. At least one light source provided for emitting a switching radiation and for emitting an excitation radiation. At least one of the light sources is arranged to radiate through the sample, and a switching and/or fluorescence excitation of the dye molecules is carried out. The switching is a photoactivation or a photodeactivation of the dye molecules. A focusing arrangement is provided for switching and/or for excitation to generate a line-like illumination region extending in a direction of illumination.
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Inventors:
Vorschensky, Ralph
Lippert, Helmut
Power, christopher
Rat, Benno
Lippert, Helmut
Power, christopher
Rat, Benno
Application Number:
JP2010546236A
Publication Date:
December 02, 2015
Filing Date:
February 03, 2009
Export Citation:
Assignee:
CARL ZEISS MICROSCOPY GMBH
International Classes:
G02B21/06; G01N21/64
Domestic Patent References:
| JP2007114542A | ||||
| JP2008542826A | ||||
| JP2006509246A |
Foreign References:
| WO2007135804A1 | ||||
| WO2004036284A1 | ||||
| WO2006127692A1 |
Attorney, Agent or Firm:
Makoto Onda
Hironobu Onda
Atsushi Honda
Hironobu Onda
Atsushi Honda