Title:
結合化学量論の決定方法
Document Type and Number:
Japanese Patent JP5830523
Kind Code:
B2
Abstract:
A method is provided for determining binding stoichiometry for the interaction between a first molecule and a second molecule forming a complex between them. Either (i) a solution having a fixed initial active concentration of the first molecule is titrated with solutions of varying active concentrations of the second molecule, and the free active concentrations of the second molecule are measured; or (ii) a solution with fixed initial concentrations of the first molecule and the second molecule is incubated, and the free active concentrations of both molecules are measured. In the first case, the binding stoichiometry can be determined from the initial concentration values of both molecules and the free concentration value(s) of the second molecule at saturation; and in the second case from the initial concentration values of both molecules and the free concentration values of both molecules. Active concentration measurements are typically performed by an interaction analysis sensor, using a calibration-free analytical format at least for the determination of active initial concentrations.
Inventors:
Carlson, Robert
Application Number:
JP2013502530A
Publication Date:
December 09, 2015
Filing Date:
March 29, 2011
Export Citation:
Assignee:
GE Healthcare Bioscience Activolag
International Classes:
G01N33/53; G01N21/27; G01N33/543
Other References:
Day ES et al,Selectivity of BAFF/BLyS and APRIL for binding to the TNF family receptors BAFFR/BR3 and BCMA,Biochemistry,2005年 2月15日,44(6),1919-1931
Chu YH et al,Using affinity capillary electrophoresis to determine binding stoichiometries of protein-ligand interactions,Biochemistry,1994年 9月 6日,33(35),10616-10621
Chu YH et al,Using affinity capillary electrophoresis to determine binding stoichiometries of protein-ligand interactions,Biochemistry,1994年 9月 6日,33(35),10616-10621
Attorney, Agent or Firm:
Arakawa Satoshi
Hirokazu Ogura
Toshihisa Kurokawa
Hirokazu Ogura
Toshihisa Kurokawa