Title:
アイソフォーム濃縮抗体調製物及びこれを得る方法
Document Type and Number:
Japanese Patent JP5838221
Kind Code:
B2
Abstract:
Herein is reported a method for producing an antibody preparation comprising the steps of a) applying a buffered solution comprising different isoforms of an antibody to a cation exchange chromatography material, b) applying a first solution with a first conductivity to the cation exchange chromatography material, whereby the antibody isoforms remain bound to the cation exchange chromatography material, and c) applying a second solution with a second conductivity to the cation exchange chromatography material and thereby obtaining the antibody preparation, whereby the conductivity of the second solution exceeds the conductivity of the first solution by not more than 10%.
Inventors:
Falkenstein, Robert
Schwendner, Klaus
Spenceberger, Bernhard
Schwendner, Klaus
Spenceberger, Bernhard
Application Number:
JP2013545269A
Publication Date:
January 06, 2016
Filing Date:
December 19, 2011
Export Citation:
Assignee:
Ef Hoffman-La Roche Akchengezel Shaft
International Classes:
C07K1/18; C07K16/00
Domestic Patent References:
| JP2005538176A | ||||
| JP2008542218A | ||||
| JP2007525412A | ||||
| JP2013500244A |
Foreign References:
| WO2009058812A1 |
Other References:
Journal of Chromatography,1993年,Vol.639,p.41-49
Attorney, Agent or Firm:
Sonoda Yoshitaka
Kobayashi Yoshinori
Kobayashi Yoshinori