Title:
キャリアペプチドフラグメント及びその利用
Document Type and Number:
Japanese Patent JP5858285
Kind Code:
B2
Abstract:
A method for transferring a foreign substance includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including either the amino acid sequence KKRTLRKNDRKKR (SEQ ID NO. 1) or an amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.
Inventors:
Toru Yoshida
Naoko Kobayashi
Niwa Mikio
Naoko Kobayashi
Niwa Mikio
Application Number:
JP2011524810A
Publication Date:
February 10, 2016
Filing Date:
July 28, 2010
Export Citation:
Assignee:
Toagosei Co., Ltd.
International Classes:
C12N15/09; A61K31/7052; A61K38/00; A61K45/00; A61K47/42; A61K47/48; A61K48/00; C07K7/08; C07K19/00
Domestic Patent References:
| JP2001199997A | 2001-07-24 | |||
| JP2004512275A | 2004-04-22 | |||
| JP2001199997A | 2001-07-24 | |||
| JP2004512275A | 2004-04-22 |
Attorney, Agent or Firm:
Makoto Abe
Michiko Oi
Michiko Oi