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Title:
ヒトパピローマウイルスの検出
Document Type and Number:
Japanese Patent JP5898831
Kind Code:
B2
Abstract:
There is provided an in vitro method of detecting human papillomavirus nucleic acid in a sample, comprising: (a) contacting said sample with forward and reverse oligonucleotide primers, wherein said primers bind to target sites in the human papillomavirus L1 gene, or the complement thereof, under conditions suitable to promote amplification of a portion of said human papillomavirus L1 gene or complement, thereby generating an amplicon; (b) contacting said amplicon with a probe, wherein the probe binds to a target site within said amplicon; and (c) detecting binding of said probe to said amplicon; wherein said forward primer binds to a target site having the sequence SEQ ID NO: 1; and wherein said reverse primer binds to a target site having the sequence SEQ ID NO: 2.

Inventors:
Corless Caroline
Guyver Marcolm
Application Number:
JP2009548750A
Publication Date:
April 06, 2016
Filing Date:
February 08, 2008
Export Citation:
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Assignee:
SECRETARY OF STATE FOR HEALTH
International Classes:
C12Q1/68; C12N15/09
Domestic Patent References:
JP2001502546A
Foreign References:
WO2006038753A1
US20050175989
Attorney, Agent or Firm:
Masaki Hirota
Seiji Ozawa
Yusaku Tokai
Kazuhiro Matsuda
Makoto Horiuchi