Title:
ポロキサマーを用いるレトロウイルス形質導入
Document Type and Number:
Japanese Patent JP6290106
Kind Code:
B2
Abstract:
The present invention relates to a method for transducing a target cell, the method comprising the step of contacting a target cell with a retroviral vector and a poloxamer having a molecular weight of 12,8 kDa to about 15 kDa. Further, the invention relates to the use of a poloxamer as defmed herein, optionally in combination with a polycationic substance as defmed herein, for transducing a target cell with a retroviral vector and a kit comprising a retroviral vector, a poloxamer as defined herein and, option ally, instructions for use.
Inventors:
Nataza Anastazov
Ines Hefich
Christian Tillion
Ines Hefich
Christian Tillion
Application Number:
JP2014559230A
Publication Date:
March 07, 2018
Filing Date:
February 28, 2013
Export Citation:
Assignee:
Helmholtz Zentrum Muenchen - Deutsches Forschungszentrum Fuer Gesundheit und Umwelt (GmbH)
International Classes:
C12N15/09; C12M1/00; C12Q1/68
Domestic Patent References:
| JP2003525613A |
Foreign References:
| WO2006086775A2 |
Other References:
Eur. J. Pharm. Biopharm.,2005年,vol.61, no.3,pp.126-133
J. Mol. Cell. Cardiol.,2003年,vol.35, no.7,pp.739-748
J. Mol. Cell. Cardiol.,2003年,vol.35, no.7,pp.739-748
Attorney, Agent or Firm:
Mitsuo Tanaka
Hiroshi Yamazaki
Kenji Tomita
Fumio Inai
Hiroshi Yamazaki
Kenji Tomita
Fumio Inai