Title:
ｈＵＴＣ増殖用栄養強化培地
Document Type and Number:
Japanese Patent JP6404227
Kind Code:
B2
Abstract:
This invention provides for methods of growing anchorage-dependent cells ( e.g. hUTC) in culture medium comprising amino acids, vitamins, salts nucleosides, insulin, transferrin, ethanolamine and sodium selenium, wherein the culture medium is supplemented with serum. The method further comprises addition of a serum-free nutrient solution comprising amino acids, vitamins, salts nucleosides, insulin, transferrin, ethanolamine and sodium selenium. The invention also provides for culture media and serum-free nutrient solutions for growing anchorage-dependent cells.
Inventors:
Batia Rabinder
Hong L. S. Crodaine
Ozturk sadeting es
Kamaraju Bencut H
Hong L. S. Crodaine
Ozturk sadeting es
Kamaraju Bencut H
Application Number:
JP2015547532A
Publication Date:
October 10, 2018
Filing Date:
December 12, 2013
Export Citation:
Assignee:
Depuy Synthes Products Incorporated
International Classes:
C12N5/0775; C12N1/00
Domestic Patent References:
| JP5502379A | ||||
| JP2005505240A | ||||
| JP2008518597A | ||||
| JP2010536357A |
Foreign References:
| US20040151709 |
Other References:
MINIMUM ESSENTIAL MEDIUM EAGLE - ALPHA MODIFICATION WITH NUCLEOSIDES,[ONLINE],2008年 4月 1日,URL,http://www.stemcell.com/~/media/Technical Resources/9/9/A/1/0/29846_PIS_1_1_0.pdf?la=en
Attorney, Agent or Firm:
Konobu Kato
Takafumi Oshima
Takafumi Oshima
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