Title:
抗体のリフォールディング方法、リフォールディングされた抗体の製造方法、リフォールディングされた抗体、及びこれらの利用
Document Type and Number:
Japanese Patent JP6453208
Kind Code:
B2
Abstract:
The present invention provides a method for refolding an antibody, a process for producing a refolded antibody, a refolded antibody, and uses thereof. The present invention provides a method for refolding an antibody in a liquid phase, the method comprising the steps of denaturing an inactive antibody binding directly or through a linker to a peptide, the peptide having an isoelectric point lower than the isoelectric point of the inactive antibody, and dispersing in a liquid phase the peptide-binding inactive antibody denatured in the step above. The present invention also provides a process for producing a refolded antibody.
Inventors:
Yoichi Kumada
Yasuyuki Ishikawa
Fujiwara Yusa
Michimasa Kishimoto
Yasuyuki Ishikawa
Fujiwara Yusa
Michimasa Kishimoto
Application Number:
JP2015500192A
Publication Date:
January 16, 2019
Filing Date:
February 03, 2014
Export Citation:
Assignee:
Kyoto University of Technology
International Classes:
C07K1/113; C07K16/00; C07K17/08; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10
Domestic Patent References:
| JP2011168505A |
Foreign References:
| WO2011102342A1 |
Other References:
Anal. Bioanal. Chem., (2010), 398, [3], p.1295-1303
Attorney, Agent or Firm:
Patent Business Corporation Saegusa International Patent Office