Title:
細胞培養システム
Document Type and Number:
Japanese Patent JP6457007
Kind Code:
B2
Abstract:
A method of producing a co-culture of intestinal epithelial cells and microbial cells, comprising providing a cell culture system comprising a fluidic device having a fluid channel connected to a fluid source, the fluid source supplying fluid to the fluid channel; and a membrane positioned within the channel. The method further comprises providing a fluid suitable for maintaining intestinal epithelial cells to the cell culture system such that the fluid contacts the intestinal epithelial cells, culturing the intestinal epithelial cellswherein the intestinal epithelial cells adhere to at least one surface of the membrane, maintaining microbial cells in the system for at least 1 day, wherein the microbial cells adhere to the surface of the viable intestinal epithelial cells, and flowing fluid continuously through the channel such that microbial cells not adhering to the intestinal epithelial cells are washed out.
Inventors:
Ingbar Donald E.
Kim Hyun Jun
Kim Hyun Jun
Application Number:
JP2017069507A
Publication Date:
January 23, 2019
Filing Date:
March 31, 2017
Export Citation:
Assignee:
President and Fellows of Harvard College
International Classes:
C12N5/071; C12N1/20
Foreign References:
| WO2010009307A1 | ||||
| US20100041128 | ||||
| WO2010118857A1 |
Other References:
Lab Chip (2008) vol.8, issue 5, p.741-746
Attorney, Agent or Firm:
Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Kazuya Kawamoto