Title:
水産生物伝染病原因ウイルスの判別及び検出用遺伝子マーカー、及びこれを利用した当該ウイルスを判別及び検出する方法
Document Type and Number:
Japanese Patent JP6464316
Kind Code:
B2
Abstract:
Genetic markers are described for discriminating or detecting viruses causing infectious aquatic organism diseases, and a method of discriminating and detecting the viruses using the same is disclosed, in which the method includes selecting and amplifying a DNA nucleotide sequence encoding a gene specific for viral hemorrhagic septicemia virus (VHSV), red sea bream iridovirus (RSIV) or infectious spleen and kidney necrosis virus (ISKNV), which is a virus causing red sea bream iridovirus disease, or Koi herpesvirus (KHV); hybridizing a peptide nucleic acid (PNA) that specifically recognizes the amplification product; controlling the temperature of the hybridization product to obtain a temperature-dependent melting curve; and discriminating the viral type or detecting whether or not fish are infected with the viral type by analyzing the obtained melting curve to determine a melting temperature.
Inventors:
Cho, Miyoung
Park, Myeongae
Ji Boyon
Fan, songton
Park, Myeongae
Ji Boyon
Fan, songton
Application Number:
JP2018506936A
Publication Date:
February 06, 2019
Filing Date:
August 24, 2016
Export Citation:
Assignee:
Republic of Korea (National Institute of Fisheries Science)
International Classes:
C12Q1/6888
Foreign References:
| KR1020150028063A | ||||
| KR1020080066301A | ||||
| KR1020140091944A |
Other References:
Veterinarni Medicina,2007年,vol.52, no,12,p.562-568
BMC Microbiol.,2005年,vol.5, 13
BMC Microbiol.,2005年,vol.5, 13
Attorney, Agent or Firm:
Makoto Ono
Kenkyo Kanayama
Mitsuaki Tsubokura
Kazuki Shigemori
Kenji Ando
Hidehiko Ichikawa
Takahiro Aoki
Yousuke Kawasaki
Takuya Gomibuchi
Toshikazu Imato
Yoichi Iino
Yusuke Ichikawa
Masahiro Moriyama
Yoshikazu Iwase
Yasufumi Shiroyama
Kenkyo Kanayama
Mitsuaki Tsubokura
Kazuki Shigemori
Kenji Ando
Hidehiko Ichikawa
Takahiro Aoki
Yousuke Kawasaki
Takuya Gomibuchi
Toshikazu Imato
Yoichi Iino
Yusuke Ichikawa
Masahiro Moriyama
Yoshikazu Iwase
Yasufumi Shiroyama