Title:
純粋な染料計器を正常化する方法およびシステム
Document Type and Number:
Japanese Patent JP6496416
Kind Code:
B2
Abstract:
The present teachings relate to a method and system for normalizing spectra across multiple instruments. In an embodiment of the present invention, the method comprises at least one reference instrument and a test instrument. Each instrument comprises at least one excitation filter and at least one emission filter arranged in pairs. Each instrument further comprises a pure dye plate comprising a plurality of wells. Each well contains a plurality of dyes where each dye comprises a fluorescent component. Fluorescent spectra are obtained from each instrument for each dye across multiple filter combinations to contribute to a pure dye matrix Mref for the reference instrument and pure dye matrix M for the test instrument. The pure dye spectra can then be multiplied by correction factors for each filter pair to result in corrected pure dye spectra, then normalized and the multicomponenting data can be extracted.
Inventors:
Marks, Jeffrey
Application Number:
JP2017541321A
Publication Date:
April 03, 2019
Filing Date:
February 05, 2016
Export Citation:
Assignee:
Life Technologies Corporation
International Classes:
G01N21/64
Domestic Patent References:
| JP2003505691A | ||||
| JP2013537635A | ||||
| JP2004535198A | ||||
| JP2008536092A | ||||
| JP2013533523A | ||||
| JP2011505004A | ||||
| JP2003508064A |
Foreign References:
| US20070100569 |
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Natsuki Morishita