Title:
全ゲノムの高速マッピング用ナノ流体デバイス、並びに関連する分析システム及び分析方法
Document Type and Number:
Japanese Patent JP6499638
Kind Code:
B2
Abstract:
Devices and methods generate an ordered restriction map of genomic DNA extracted from whole cells, nuclei, whole chromosomes, or other sources of long DNA molecules. The devices have a fluidic microchannel that merges into a reaction nanochannel that merges into a detection nanochannel at an interface where the nanochannel diameter decreases in size by between 50% to 99%. Intact molecules of DNA are transported to the reaction nanochannel and then fragmented in the reaction nanochannel using restriction endonuclease enzymes. The reaction nanochannel is sized and configured so that the fragments stay in an original order until they are injected into the detection nanochannel. Signal at one or more locations along the detection nanochannel is detected to map fragments in the order they occur along a long DNA molecule.
Inventors:
Ramsey, John Michael
Menard, Laurent
Menard, Laurent
Application Number:
JP2016501220A
Publication Date:
April 10, 2019
Filing Date:
March 11, 2014
Export Citation:
Assignee:
THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
International Classes:
C12M1/00; C12Q1/68; G01N27/447; G01N33/48; G01N33/483; G01N37/00
Domestic Patent References:
| JP10507516A | ||||
| JP2004271533A | ||||
| JP2003511699A |
Foreign References:
| US20090023146 | ||||
| US20060084078 |
Other References:
PNAS (2005) Vol.102, No.29, pp.10012-10016
ACS NANO (2012) Vol.6, No.10, pp.9087-9094
ACS NANO (2012) Vol.6, No.10, pp.9087-9094
Attorney, Agent or Firm:
Tatsuya Saito