Title:
操作されたボツリヌス神経毒素
Document Type and Number:
Japanese Patent JP6574218
Kind Code:
B2
Abstract:
Disclosed herein are botulinum neurotoxin (BoNT) polypeptides with a modified receptor binding domain of Clostridial botulinum serotype B (B-H c ), comprising one or more substitution mutations corresponding to substitution mutations in serotype B, strain 1, V1118M; Y1183M; E1191M; E1191I; E1191Q; E1191T; S1199Y; S1199F; S1199L; S1201V; or combinations thereof. Specific combination mutations include E1191M and S1199L, E1191M and S1199Y, E1191M and S1199F, E1191Q and S1199L, E1191Q and S1199Y, or E1191Q and S1199F. Other substitution mutations are also disclosed. Isolated modified receptor binding domains, chimeric molecules, pharmaceutical compositions, and methods of using the same are also disclosed.
Inventors:
Don Min
Pen Richen
Stainless Mark Paul Eric Gustav
Bartson Ronnie Pell-Arne
Pen Richen
Stainless Mark Paul Eric Gustav
Bartson Ronnie Pell-Arne
Application Number:
JP2017095111A
Publication Date:
September 11, 2019
Filing Date:
May 12, 2017
Export Citation:
Assignee:
President and Fellows of Harvard College
International Classes:
C12N15/31; A61K35/74; A61K38/16; A61K48/00; A61P11/00; A61P11/06; A61P19/00; A61P25/02; A61P25/04; C07K14/33; C07K19/00
Domestic Patent References:
| JP2008538902A |
Other References:
Nature,2006年,Vol.444,p.1092-1095
Proc Natl Acad Sci USA,2007年,Vol.104, No.1,p.359-364
Nature,2006年,Vol.444,p.1096-1100
Proc Natl Acad Sci USA,2007年,Vol.104, No.1,p.359-364
Nature,2006年,Vol.444,p.1096-1100
Attorney, Agent or Firm:
Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Kazuya Kawamoto