Title:
貪食能評価方法及び蛍光測定方法
Document Type and Number:
Japanese Patent JP6600527
Kind Code:
B2
Abstract:
The present invention relates to a method for evaluating phagocytic capacity of phagocytes, including incorporating a fluorescent substance into phagocytes in a sample containing whole blood to obtain a measurement sample, standing the measurement sample for 2 minutes or more, measuring the fluorescence of the fluorescent substance, and evaluating the phagocytic capacity of phagocytes based on the intensity of the measured fluorescence, in which the measuring the fluorescence of the fluorescent substance includes irradiating an intermediate region of gravity direction of a placed measurement sample with an excitation light, and detecting the fluorescence generated from the fluorescent substance by the excitation light.
Inventors:
Fire
Hiroyuki Inagawa
Genichiro Sou
Kimiko Kimmura
Koji Tsuchiya
Morishita Naoto
Hiroyuki Inagawa
Genichiro Sou
Kimiko Kimmura
Koji Tsuchiya
Morishita Naoto
Application Number:
JP2015203488A
Publication Date:
October 30, 2019
Filing Date:
October 15, 2015
Export Citation:
Assignee:
Innate immune control technology research association
Hamamatsu Photonics Co., Ltd.
Hamamatsu Photonics Co., Ltd.
International Classes:
C12Q1/06; G01N21/64
Domestic Patent References:
| JP2010508295A | ||||
| JP2012073109A |
Other References:
Schreiner, L., et al.,Phagocytosis and digestion of pH-sensitive fluorescent dye (Eos-FP) transfected E. coli in wohle blood assays from patients with severe sepsis and septic shock,J. Cell Commun. Signal.,2011年,Vol.5,p.135-144
Attorney, Agent or Firm:
Yoshiki Hasegawa
Yoshiki Kuroki
Kenichi Shibayama
Toshiaki Sakanishi
Yoshiki Kuroki
Kenichi Shibayama
Toshiaki Sakanishi