Title:
ＸＴＥＮおよびｖｏｎＷｉｌｌｅｂｒａｎｄ因子タンパク質を有する第ＶＩＩＩ因子の複合体、および、その使用
Document Type and Number:
Japanese Patent JP6603128
Kind Code:
B2
Abstract:
The present invention provides a chimeric protein comprising a VWF protein comprising the D' domain and D3 domain of VWF, one or more XTEN sequence, and a FVIII protein, wherein the VWF fragment, the XTEN sequence, or the FVIII protein are linked to or associated with each other. The chimeric protein can further comprise one or more Ig constant region or a portion thereof (e.g., an Fc region). A polypeptide chain comprising a VWF fragment of the invention binds to or is associated with a polypeptide chain comprising a FVIII protein linked to an XTEN sequence and the polypeptide chain comprising the VWF fragment can prevent or inhibit binding of endogenous VWF to the FVIII protein linked to the XTEN sequence. By preventing or inhibiting binding of endogenous VWF to the FVIII protein, which is a half-life limiting factor for FVIII, the VWF fragment can induce extension of half-life of the chimeric protein comprising a FVIII protein. The invention also includes nucleotides, vectors, host cells, methods of using the VWF fragment, or the chimeric proteins.
Inventors:
Chabla, Ectacess
Riu, Dong Yao
Chang, Pay-Yoon
Peters, Robert Tee.
Courman, John
Riu, Dong Yao
Chang, Pay-Yoon
Peters, Robert Tee.
Courman, John
Application Number:
JP2015521785A
Publication Date:
November 06, 2019
Filing Date:
July 10, 2013
Export Citation:
Assignee:
Bioverative Therapeutics, Inc.
International Classes:
C12N15/12; A61K35/12; A61K35/76; A61K38/36; A61K48/00; A61P7/04; A61P43/00; C07K14/755; C07K19/00; C12N5/10; C12P21/02
Other References:
Nature Biotechnology,2009年,Vol.27,No.12,pp.1186-1190
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto