Title:
肝代謝物の毛細胆管様構造への蓄積と排泄を促進する肝細胞培養装置、並びに該肝細胞培養装置を用いた胆汁中又は血液中排泄感受性の候補化合物及び該候補化合物の肝代謝物の評価方法
Document Type and Number:
Japanese Patent JP6704604
Kind Code:
B2
Abstract:
The present invention is a hepatocyte culture device enhancing accumulation and excretion of a hepatic metabolite in and into a bile canaliculus-like structure, the device including: a first cell culture containing a plurality of hepatocytes; a portable culture vessel in which the bile canaliculus-like structure can be established between the hepatocytes in the first cell culture, and that has, on an outer surface of the culture vessel, a membrane through which a physiologically active substance can pass; and a second cell culture capable of improving excretion activity of a hepatic metabolite in the first cell culture, in which the first cell culture cultured in the portable culture vessel is disposed on the second cell culture to be co-cultured.
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Inventors:
Toshiaki Takezawa
Application Number:
JP2017509535A
Publication Date:
June 03, 2020
Filing Date:
March 16, 2016
Export Citation:
Assignee:
National Institute for Agricultural and Food Industry Research Organization
International Classes:
C12Q1/02; G01N33/15; G01N33/50
Domestic Patent References:
| JP2007510429A | ||||
| JP2008503204A | ||||
| JP2003502016A |
Foreign References:
| WO2009099066A1 | ||||
| WO2007037407A1 | ||||
| WO2011024592A1 | ||||
| WO2010062911A1 | ||||
| WO2013086502A1 |
Other References:
押方歩ほか,コラーゲンビトリゲル膜チャンバーを利用した新しい肝代謝試験法の開発,日本薬学会年会要旨集,2014年, vol.134,S44-3
NAGAMOTO, Y., et al.,Biomaterials,2012年,vol.33, issue 18,p.4526-4534
SAKAI, Y., et al.,Cytotechnology,1996年,vol.21, no.3,p.243-252
NAGAMOTO, Y., et al.,Biomaterials,2012年,vol.33, issue 18,p.4526-4534
SAKAI, Y., et al.,Cytotechnology,1996年,vol.21, no.3,p.243-252
Attorney, Agent or Firm:
Nishizawa Kazumi
Masato Iida
Taichi Sakai
Masato Iida
Taichi Sakai