Title:
膜結合蛋白質を結合させたゴム粒子の製造方法、空気入りタイヤの製造方法及びゴム製品の製造方法
Document Type and Number:
Japanese Patent JP6706813
Kind Code:
B2
Abstract:
The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.
Inventors:
Haruhiko Yamaguchi
Yukino Inoue
Kazuhisa Fushihara
Seiji Takahashi
Satoshi Yamashita
Toru Nakayama
Yuzuru Tozawa
Yukino Inoue
Kazuhisa Fushihara
Seiji Takahashi
Satoshi Yamashita
Toru Nakayama
Yuzuru Tozawa
Application Number:
JP2015131023A
Publication Date:
June 10, 2020
Filing Date:
June 30, 2015
Export Citation:
Assignee:
Sumitomo Rubber Industries, Ltd.
Tohoku University
Tohoku University
International Classes:
C08G81/02; B29D30/00; C12N15/09
Domestic Patent References:
| JP2009221306A | ||||
| JP2011052146A | ||||
| JP2014227487A | ||||
| JP2011188776A | ||||
| JP2005308412A | ||||
| JP2003310295A | ||||
| JP2003018999A |
Other References:
Eur. J. Biochem., 2003, Vol.270, p.4671-4680
横山茂之,細胞を使わないで、膜タンパク質合成系を開発,Nature Asia-Pacific,2010年,Vol.7/No.4-5,28-29
K.Asawatreratanakul, Y.-W.-Zhang, D.Wititsuwannakul, R.Wititsuwannakul, S.Takahashi, A.Rattanapittay,Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Heve,Eur. J. Biochem.,2003年,Vol.270,4671-4680
内海俊彦,無細胞タンパク質合成系を用いた脂質修飾タンパク質の網羅的探索と機能解析,生化学,日本,2010年,第82巻、第9号,799-813
横山茂之,細胞を使わないで、膜タンパク質合成系を開発,Nature Asia-Pacific,2010年,Vol.7/No.4-5,28-29
K.Asawatreratanakul, Y.-W.-Zhang, D.Wititsuwannakul, R.Wititsuwannakul, S.Takahashi, A.Rattanapittay,Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Heve,Eur. J. Biochem.,2003年,Vol.270,4671-4680
内海俊彦,無細胞タンパク質合成系を用いた脂質修飾タンパク質の網羅的探索と機能解析,生化学,日本,2010年,第82巻、第9号,799-813
Attorney, Agent or Firm:
Atomi International Patent Office