Title:
高親水性高分子モノマーを主剤とする電子顕微鏡用包埋方法
Document Type and Number:
Japanese Patent JP6721899
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a new embedding agent using a highly hydrophilic resin, which serves as a substitute for formalin as a base material, and to provide an embedding method for electron microscope using the embedding agent.SOLUTION: A tissue embedding method includes immersing a tissue in an embedding liquid, and standing and polymerizing the liquid, where the embedding liquid is obtained by adding, to 100 pts.wt. of an aqueous solution, 1-5 pts.wt. of a crosslinking agent, 0.0001-0.001 pt.wt. of a polymerization accelerator and, as other monomer(s), 0-100 pts.wt. of one or more monomers selected from the group consisting of methyl methacrylate, hydroxyethyl methacrylate, dodecyl methacrylate, 2-methyl-2-propenoic acid Calkyl ester, and (1-methylethylidene)bis(4,1-phenyleneoxy-2,1-ethanediyl)bismethacrylate, the aqueous solution containing N-vinyl-2-pyrrolidone (NVP), which has high hydrophilicity and becomes a polymer when polymerized, in a monomer concentration of 98 to 100%.SELECTED DRAWING: None
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Inventors:
Hirokazu Kitamura
Aoi Ito
Yoshihisa Fujikura
Aoi Ito
Yoshihisa Fujikura
Application Number:
JP2016069228A
Publication Date:
July 15, 2020
Filing Date:
March 30, 2016
Export Citation:
Assignee:
National University Corporation Oita University
International Classes:
C08F26/10; G01N1/36; G01N1/28; G01N33/48; H01J37/20
Domestic Patent References:
| JP2004085219A | ||||
| JP2015533210A | ||||
| JP2000346770A | ||||
| JP2009191125A | ||||
| JP2006036957A | ||||
| JP2006519376A | ||||
| JP2002340900A | ||||
| JP7006893B2 |
Foreign References:
| WO2013035681A1 |
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Masaru Nakajima
Ryotaro Takei
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Masaru Nakajima
Ryotaro Takei