Title:
乳汁中のストレプトコッカス属の菌を検出する方法
Document Type and Number:
Japanese Patent JP6735315
Kind Code:
B2
Abstract:
The object is to provide a lysis method and lysis treatment solution for efficiently lysing cells of various Streptococcus bacteria in milk of a livestock animal to release a specific antigen substance contained in the cells for detecting whether causative bacterium of mastitis is a Streptococcus bacterium or not by using the milk, as well as a detection method using an immunochromatographic device. There is provided a method for lysing a Streptococcus bacterium, which comprises the step of mixing a lysis agent containing a lytic enzyme with milk obtained form a livestock animal to lyse a Streptococcus bacterium existing in the milk. The lytic enzyme is preferably at least one selected from the group consisting of lysozyme, labiase, and ²-N-acetylglucosaminidase.
Inventors:
Takamitsu Utsumi
Hiroshi Maehana
Kenji Matsuyama
Hiroshi Maehana
Kenji Matsuyama
Application Number:
JP2018106025A
Publication Date:
August 05, 2020
Filing Date:
June 01, 2018
Export Citation:
Assignee:
ASAHI KASEI KABUSHIKI KAISHA
International Classes:
C12N1/06; C12N1/20; C12Q1/04; C12Q1/34; G01N33/48; G01N33/543; G01N33/569
Domestic Patent References:
| JP2012122921A |
Other References:
Sentitula et al.,Indian J Microbiol,2012年,Vol.52, No.2,p.153-159
B.E.Gillespie and S.P.Oliver ,J.Dairy Sci.,2005年,Vol.88,p.3510-3518
Kozue Oana et al.,Microbiol Immunol,2009年,Vol.53,p.45-48
B.E.Gillespie and S.P.Oliver ,J.Dairy Sci.,2005年,Vol.88,p.3510-3518
Kozue Oana et al.,Microbiol Immunol,2009年,Vol.53,p.45-48
Attorney, Agent or Firm:
Patent Service Corporation Patent Office Sykes