Title:
多能性幹細胞から生殖細胞への分化誘導方法
Document Type and Number:
Japanese Patent JP6757980
Kind Code:
B2
Abstract:
The invention provides a method for inducing human primordial germ cell-like (PGC-like) cells from human pluripotent stem cells, with high efficiency and high reproducibility, and a cell surface marker for identifying human PGC-like cells. In particular, the invention provides a method for producing a human PGC-like cell from a human pluripotent stem cell, includes a step of producing a mesoderm-like cell by culturing a human pluripotent stem cell in a culture medium comprising activin A and a GSK3β inhibitor, and a step of culturing the mesoderm-like cell in a culture medium containing BMP. The invention also provides a method for producing an isolated human PGC-like cell, which includes the aforementioned two steps and the additional step of selecting a cell positive to at least one cell surface marker selected from the group consisting of PECAM (CD31), INTEGRINa6 (CD49f), INTEGRINβ3 (CD61), KIT (CD117), EpCAM, PODOPLANIN and TRA1-81.
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Inventors:
Miki Saito
Kotaro Sasaki
Yokobayashi Shihori
Kotaro Sasaki
Yokobayashi Shihori
Application Number:
JP2017526415A
Publication Date:
September 23, 2020
Filing Date:
June 29, 2016
Export Citation:
Assignee:
Kyoto University
International Classes:
C12N5/071; C12N5/10
Domestic Patent References:
| JP2013530680A | ||||
| JP2013538038A |
Foreign References:
| WO2014174470A1 |
Other References:
Cell,2015年 1月15日,Vol.160,p.253-268
EMBO J.,2015年 3月 6日,Vol.34, No.8,p.1009-1024
Fertility and Sterility,2014年 9月,Vol.102, No.3,SUPPL.1, p.e103, O-303
EMBO J.,2015年 3月 6日,Vol.34, No.8,p.1009-1024
Fertility and Sterility,2014年 9月,Vol.102, No.3,SUPPL.1, p.e103, O-303
Attorney, Agent or Firm:
Takashima Ichi
Kyoko Doi
Mitsunori Kamata
Yaeko Tamura
Junzo Koike
Hirofumi Toma
Atsuko Akai
Tomiya Tozaki
Koichiro Muraoka
Kyoko Doi
Mitsunori Kamata
Yaeko Tamura
Junzo Koike
Hirofumi Toma
Atsuko Akai
Tomiya Tozaki
Koichiro Muraoka