Title:
多重特異性分子の機能分析のためのＳＰＲに基づく二重結合アッセイ
Document Type and Number:
Japanese Patent JP6785372
Kind Code:
B2
Abstract:
Herein is reported a method for determining the binding of an antibody, which comprises a first binding site specifically binding to a first antigen and a second binding site specifically binding to a second antigen, to said first and said second antigen, wherein the method comprises the steps of capturing the antibody on a solid phase using a capture reagent specifically binding to a constant domain of the antibody, incubating the captured antibody with the first or the second antigen to form a captured antibody-antigen complex and determining a first binding signal, either i) incubating the captured antibody-antigen complex with the antigen not used for the formation of the captured antibody-antigen complex to form a captured antibody-antigen-antigen complex and determining a second binding signal, or ii) regenerating the surface, capturing the antibody on a solid phase using a capture reagent specifically binding to a constant domain of the antibody, incubating the captured antibody with the antigen not used for the formation of the captured antibody-antigen complex in step b) to form a captured antibody-antigen-antigen complex and determining a third binding signal, and determining the overall or individual binding of the antibody to the first and the second antigen from the first binding signal and the second or third binding signal.
Inventors:
Gusner Christian
Meschendelfer Wolfgang
Melleken Jörg
Meschendelfer Wolfgang
Melleken Jörg
Application Number:
JP2019517380A
Publication Date:
November 18, 2020
Filing Date:
September 21, 2017
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
G01N33/53; G01N21/41; G01N33/543
Domestic Patent References:
| JP2014228385A | ||||
| JP2007524098A | ||||
| JP2012522493A | ||||
| JP2005510696A |
Foreign References:
| WO2015086549A1 | ||||
| US20010024795 |
Other References:
W. Meshendoerfer,SPR-based assays enable the full functional analysis of bispecific molecules,Journal of Pharmaceutical and Biomedical Analysis,2016年 9月26日,Vol.132,Page.141-147
Attorney, Agent or Firm:
Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto