Title:
確率的配列決定プロセスのための塩基呼出
Document Type and Number:
Japanese Patent JP6814875
Kind Code:
B2
Abstract:
Techniques for measuring sequences of nucleic acids are provided. Time-based measurements (e.g., forming a histogram) particular to a given sequencing cell can be used to generate a tailored model. The model can include probability functions, each corresponding to different states (e.g., different states of a nanopore). Such probability functions can be fit to a histogram of measurements obtained for that cell. The probability functions can be updated over a sequencing run of the nucleic acid so that drifts in physical properties of the sequencing cell can be compensated. A hidden Markov model can use such probability functions as emission probabilities for determining the most likely nucleotide states over time. For sequencing cells involving a polymerase, a 2-state classification between bound and unbound states of the polymerase can be performed. The bound regions can be further analyzed by a second classifier to distinguish between states corresponding to different bound nucleotides.
Inventors:
Major, Morgan
John the Manion
John the Manion
Application Number:
JP2019506441A
Publication Date:
January 20, 2021
Filing Date:
August 04, 2017
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
G16B30/00; G16B40/20; G16B45/00
Foreign References:
| WO2016099673A1 | ||||
| US20160203196 |
Attorney, Agent or Firm:
Shinjiro Ono
Osamu Yamamoto
Toru Miyamae
Motoharu Nakanishi
Osamu Yamamoto
Toru Miyamae
Motoharu Nakanishi