Title:
乳酸酸化酵素、乳酸酸化酵素をコードする核酸分子、乳酸酸化酵素を用いた乳酸測定方法、乳酸センサー、及びバイオ燃料電池
Document Type and Number:
Japanese Patent JP6816536
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide lactate oxidase different from known lactate oxidase, in particular, to provide lactate oxidase having high affinity for low concentration lactate, and to provide technology utilizing physicochemical properties of lactate oxidase.SOLUTION: A lactate oxidase has lactate oxidation activity and acts on L-lactate in the presence of oxygen to produce pyruvate and hydrogen peroxide, and has any of the following amino acid sequences (a) to (c). (a) An amino acid sequence indicated by sequence number 2. (b) The amino acid sequence indicated by sequence number 2 in which the amino acid sequence is modified to include at least one of an amino acid deletion, substitution, insertion and addition for one or multiple amino acids. (c) An amino acid sequence having at least 95% sequence identity as the amino acid sequence indicated by sequence number 2. Also provided are a large scale production technology of lactate oxidase by a genetic engineering method, a lactate measurement method utilizing lactate oxidase, a lactic acid sensor, and a biofuel cell.SELECTED DRAWING: Figure 9
Inventors:
Koji Shigemori
Application Number:
JP2017014636A
Publication Date:
January 20, 2021
Filing Date:
January 30, 2017
Export Citation:
Assignee:
Aisin Seiki Co., Ltd.
International Classes:
C12N15/53; C12M1/34; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12Q1/26; G01N27/327; G01N27/416; H01M4/90; H01M8/16
Domestic Patent References:
| JP2010271154A | ||||
| JP10248574A | ||||
| JP2015525064A | ||||
| JP2013066466A |
Attorney, Agent or Firm:
Patent business corporation r&c