Title:
多孔質支持体上の細胞への大きなカーゴの効率的な送達
Document Type and Number:
Japanese Patent JP6839073
Kind Code:
B2
Abstract:
In various embodiments, method and devices for delivering large cargos (e.g., organelles, chromosomes, bacteria, and the like) into cells are provided. In certain embodiments method of delivering a large cargo into eukaryotic cells, are provided that involve providing eukaryotic cells disposed on one side of a porous membrane; providing the cargo to be delivered in a solution disposed in a reservoir chamber on the opposite side of the porous membrane; and applying pressure to the reservoir chamber sufficient to pass the cargo through pores comprising said porous membrane wherein said cargo passes through cell membranes and into the cells.
Inventors:
Tin-Cyan Wu
Pay-You Echo
Michael A. Tatel
Pay-You Echo
Michael A. Tatel
Application Number:
JP2017502931A
Publication Date:
March 03, 2021
Filing Date:
March 26, 2015
Export Citation:
Assignee:
The Regents of the University of California
International Classes:
C12N15/09; C12M1/00
Other References:
14th international conference on miniaturized systems for chemistry and life sciences,2010年,p.491-493
Attorney, Agent or Firm:
Yasuhiko Murayama
Shinya Mitsuhiro
Tatsuhiko Abe
Shinya Mitsuhiro
Tatsuhiko Abe