Title:
タンパク質のＰＥＧ化に用いるための添加物系
Document Type and Number:
Japanese Patent JP6921821
Kind Code:
B2
Abstract:
The present disclosure provides an additive system for use in protein PEGylation. The additive system includes />aminobenzoic hydrazide used either alone or in combination with aromatic amines, such as 3,5-diaminobenzoic acid, or with ammonium sals such as ammonium chloride or ammonium acetate. The disclosed additive combination provides several benefits including increased reaction rates, higher yields and reduction in the aminoxy-PEG equivalents required to complete the conjugation reaction. Typical reactions can be run by combining the additive or additive system with a solution of a protein and aminoxy-PEG reagent. The solution is adjusted to pH 4 and held at 20-25 °C without stirring until completion, typically within 24 hours.
Inventors:
Matthew Earl Hicky
Antonio Ramirez
Antonio Ramirez
Application Number:
JP2018526713A
Publication Date:
August 18, 2021
Filing Date:
November 22, 2016
Export Citation:
Assignee:
BRISTOL-MYERS SQUIBB COMPANY
International Classes:
C07K1/10; C07K1/08; C07K14/50
Domestic Patent References:
| JP2009515881A |
Attorney, Agent or Firm:
Norihito Yamao
Nagatoshi Shinagawa
Kure Hidenori
Kamahira Futami
Mizuhara Masahiro
Nagatoshi Shinagawa
Kure Hidenori
Kamahira Futami
Mizuhara Masahiro