Title:
高機能分化誘導細胞の濃縮方法及び高機能分化誘導細胞集団
Document Type and Number:
Japanese Patent JP6954531
Kind Code:
B2
Abstract:
To provide methods for selecting and concentrating highly functional differentiation-induced cells from a pluripotent stem cell-derived differentiation-induced cell group, and additionally to provide a highly functional differentiation-induced cell population concentrated by the concentration method.SOLUTION: The present invention provides a method by introducing a drug resistance gene into the gene locus of drug metabolizing enzyme CYP of pluripotent stem cells. Highly functional differentiation-induced cells, specifically, CYP high expression differentiation-induced hepatocytes and CYP high expression differentiation-induced small intestinal epithelial cells can be selected and concentrated by subjecting pluripotent stem cells in which the drug resistance gene is induced to differentiation-inducing treatment and by culturing obtained differentiation-induced cells in the presence of the drug corresponding to the drug resistance. It was confirmed that a highly functional differentiation-induced hepatocyte population obtained by concentration expresses CYP3A4, CYP1A2, CYP2C19, and the like which are markers of mature hepatocytes, equally to human primary culture hepatocytes, and a highly functional differentiation-induced small intestinal epithelial cell population expresses Villin, ISX, ANPEP, and the like which are small intestinal epithelial cell markers, equally to human small intestinal cells.SELECTED DRAWING: Figure 21
Inventors:
Hiroyuki Mizuguchi
Kazuo Takayama
Kazuo Takayama
Application Number:
JP2017177607A
Publication Date:
October 27, 2021
Filing Date:
September 15, 2017
Export Citation:
Assignee:
National University Corporation Osaka University
National Research and Development Agency Pharmaceutical Infrastructure, Health and Nutrition Institute
National Research and Development Agency Pharmaceutical Infrastructure, Health and Nutrition Institute
International Classes:
C12N5/10; A61L27/38; C12Q1/02
Domestic Patent References:
| JP2016503304A |
Foreign References:
| WO2016147975A1 |
Other References:
The FASEB Journal,2020年,Vol.34,p9141-9155
Biomaterials,2018年01月18日,Vol.161,p24-32
Biomaterials,2018年01月18日,Vol.161,p24-32
Attorney, Agent or Firm:
Takashi Shoji
Yuriko Shinobu
Takuya Osugi
Yuriko Shinobu
Takuya Osugi