Document Type and Number:
Japanese Patent JPH0120871
Kind Code:
B2
Abstract:
The process for producing L-threonine consists in that there is cultivated a producer of L-threonine in the capacity of which is used the Escherichia coli strain VNIIgenetika M-1 deposited in the Central museum of commercial microorganisms under the All-Union Research Institute for Genetics and Selection of Commercial Microorganisms at a registration No. IIMIIB-1856. The above strain has been selected on the basis of natural variability of the Escherichia coli strain VNIIgenetika VL 334/p YN7), obtained by virtue of the genetic engineering techniques through increasing the dose of mutant genes capable of a higher rate of L-threonine production, by introducing a multicopy hydrid plasmid carrying said genes, into a mutant recipient strain. The abovesaid producer is cultivated on a nutrient medium, containing sources of carbon, nitrogen, and some mineral salts in the presence of an antibiotic penicillin, the resultant biomass being then separated from the culture fluid, whereupon the end product is isolated.
Inventors:
URAJIMIIRU GEORUGIEUITSUCHI DEBABOFU
NERI ISAAKOBUNA ZUDANOWA
AREKUSANDORU KONSUTANCHINOITSUCHI SOKOROFU
BITARII ARUKADEIEUITSUCHI RIUSHITSU
YURII IWANOITSUCHI KOZUROFU
EFUGENII MOISEEEUITSUCHI KURUGE
NERI ISAAKOBUNA ZUDANOWA
AREKUSANDORU KONSUTANCHINOITSUCHI SOKOROFU
BITARII ARUKADEIEUITSUCHI RIUSHITSU
YURII IWANOITSUCHI KOZUROFU
EFUGENII MOISEEEUITSUCHI KURUGE
Application Number:
JP5773380A
Publication Date:
April 18, 1989
Filing Date:
April 30, 1980
Export Citation:
Assignee:
FUSESO* NAUCHINO ISUSUREDO INST GENECHIKI I SEREKUTSUII PUROMU* MIKUROORUGANIZUMOFU BUNIIGENECHIKA
International Classes:
C12N15/09; C07H21/04; C12N1/20; C12N15/52; C12P13/08; C12P19/34; C12R1/19
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