- Patent JPH0476997

Document Type and Number:

Japanese Patent JPH0476997

Kind Code:

B2

Abstract:

Compounds having formula (I), wherein T is a polycycloalkylidene group (e.g., adamant-2-ylidene); R is a C1-20 alkyl, aralkyl or cycloalkyl group; and Y is a fluorescent chromophore (e.g., m-phenylene), produced by reacting a compound having formula (II) with an R-ylating agent (e.g., R2SO4) in the presence of an alkali metal alkoxide in a polar aprotic solvent. Also, compounds having formula (III) are produced by reacting a compound having formula (IV) with formula (V), wherein X is an electronegative leaving group (e.g., a halogen anion such as chloride ion) in the presence of a Lewis base (e.g., a trialkyl-amine) dissolved in an aprotic organic solvent (e.g., benzene or toluene). Also, compounds having formula (VI) are produced by reacting a compound of formula (VII) with a tetra-O-acylated-O-hexopyranoside halide, then hydrolysing off the protective acyl groups. The aforementioned compounds and procedures are useful in the synthesis of enzyme-cleavable 1,2-dioxetane ring systems that can serve as members of a binding pair employed, for example, in chemiluminescent immunoassays, DNA probe assays, and direct assays for an enzyme.

Inventors:

IREENA WAI BURONSUTAIN
BURUTSUKUSU EDOWAAZU

Application Number:

JP3359890A

Publication Date:

December 07, 1992

Filing Date:

February 14, 1990

Export Citation:

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Assignee:

TROPIX INC

International Classes:

B01J31/02; C07B61/00; C07C41/00; C07C41/01; C07C43/178; C07C43/196; C07C43/215; C07C45/00; C07C49/84; C07C67/00; C07C69/00; C07C69/145; C07C69/157; C07C241/00; C07C251/30; C07D321/00; C07F9/12; C07F9/655; C07F9/6574; C07H15/203; C07H15/24; C07H15/26; C09K11/06; C09K11/07; C12Q1/34; C12Q1/42; C12Q1/44; C12Q1/68; G01N21/76; G01N33/50; G01N33/535; G01N33/58

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