Title:
【発明の名称】還元的にグリコシル化されたN―末端アミノ酸に向けられた抗原を使用するグリコシル化されたタンパクの免疫学的測定法
Document Type and Number:
Japanese Patent JPH05500111
Kind Code:
A
Abstract:
An immunoassay for a protein that is non-enzymatically glycosylated on the alpha amino group of its N-terminal amino acid, reacting a sample containing the glycosylated protein with a reducing agent; contacting the reduced glycosylated protein with an antibody directed to Glc-ol-X which is prepared by immunizing an animal with an immunogen of the formula (Glc-ol-X-L)n-carrier; and detecting or quantitating the reduced glycosylated protein bound to the antibody. In formula (Glc-ol-X-L)n-carrier: X is the N-terminal amino acid of the glycosylated protein, except that X cannot be lysine; L is a bond or a linker group; Glc-ol is the reduced form of the sugar attached to X on the glycosylated protein, and Glc-ol is attached to the alpha amino group of X; the carrier is an immunogenic compound other than the glycosylated protein; and n is from 1 to the number of available coupling sites on the carrier.
Inventors:
Anderson Byron Yee
Divis Lyman Yi
Divis Lyman Yi
Application Number:
JP51251690A
Publication Date:
January 14, 1993
Filing Date:
August 17, 1990
Export Citation:
Assignee:
Northwestern University
International Classes:
C07K1/113; C07K14/00; C07K16/00; C07K14/76; C07K16/18; C07K19/00; G01N21/31; G01N33/53; G01N33/535; G01N33/66; G01N33/68; G01N33/72; (IPC1-7): C07K15/12; G01N33/53; G01N33/535
Attorney, Agent or Firm:
Minoru Nakamura (7 outside)