Title:
【発明の名称】オリゴヌクレオチドの酵素的合成
Document Type and Number:
Japanese Patent JPH05502594
Kind Code:
A
Abstract:
The present invention makes possible the catalytic production of sequence specific oligonucleotides through the use of a specially designed template sequence. The reaction can be made to proceed isothermally in the presence of an excess of nucleoside triphosphates, an agent for polymerization, and a cutting agent. Because the process is catalytic with respect to the template sequence, an unlimited amount of oligonucleotide product can theoretically be generated from a single molecule of template. Where the process is initiated by the presence of a nucleic acid target sequence, the method of the present invention can be used for diagnostic purposes as an amplification method to improve sensitivity. Diagnostic sensitivity can be further enhanced by employing a cascade of these template sequences.
More Like This:
Inventors:
Richards, Rodney M
Application Number:
JP51755791A
Publication Date:
May 13, 1993
Filing Date:
September 23, 1991
Export Citation:
Assignee:
Amjien Incorporated
International Classes:
A61K31/70; C12N15/09; C07H1/00; C07H15/12; C07H21/00; C12N15/10; C12P19/34; C12Q1/68; G01N33/566; G01N33/58; A61K; C07H; C12P; C12Q; G01N; (IPC1-7): C12N15/10; C12Q1/68
Attorney, Agent or Firm:
Yoshio Kawaguchi (4 outside)