Title:
【発明の名称】組換え体タンパク質の回収方法
Document Type and Number:
Japanese Patent JPH05503212
Kind Code:
A
Abstract:
A method for recovering a recombinant protein from a protein solution containing high molecular weight contaminating proteins by directly adding Group IIA metal salts to the solution in amounts sufficient to selectively precipitate the high molecular weight protein contaminants is disclosed. The high molecular weight precipitates are removed and the solution is further processed to remove low molecular weight contaminating proteins and other non-protein contaminants. The recombinant protein is subsequently recovered and further processed to produce a protein composition suitable for its intended use.
Inventors:
Bram, Galena
Yan, render
Lee, Yun Kay
Yan, render
Lee, Yun Kay
Application Number:
JP51418290A
Publication Date:
June 03, 1993
Filing Date:
October 01, 1990
Export Citation:
Assignee:
Pitman-Moore Incorporated
International Classes:
C07K14/575; C07K1/30; C07K14/61; C12N1/06; C12N15/09; C12P21/00; C12P21/02; C12R1/19; (IPC1-7): C07K3/24; C12N15/18; C12P21/00; C12P21/02
Attorney, Agent or Firm:
Hiroaki Tazawa (2 outside)