Title:
【発明の名称】DNAベクタ-とその組換えDNA技術への使用
Document Type and Number:
Japanese Patent JPH0634721
Kind Code:
B2
Abstract:
The invention provides a new class of DNA vectors, each comprising two replication systems; a first origin of replication resulting in a low copy number and stable inheritance of the plasmid, and a second, high copy number, origin of replication at which replication is directly controllable such that, when host cells carrying the vector are propagated under a first set of conditions, replication takes place mainly from the low copy number origin, and that when said cells are propagated under a second set of conditions, replication takes place also from the high copy number origin to produce a high yield of gene product. The controllable origin of replication may be under the control of a natural promoter of RNA transcription or a substitute promoter such as the PL promoter or lac promoter.
Inventors:
YARANTON JOFUREI TOOMASU
HANFURIIZU GUINFUOO OOEN
ROBINSON MAACHIN KIMU
KAURUKOTSUTO SERIA AN
RAITO EDOINA MERII
HANFURIIZU GUINFUOO OOEN
ROBINSON MAACHIN KIMU
KAURUKOTSUTO SERIA AN
RAITO EDOINA MERII
Application Number:
JP50167084A
Publication Date:
May 11, 1994
Filing Date:
March 23, 1984
Export Citation:
Assignee:
SERUTETSUKU LTD
International Classes:
C12N9/52; C12N9/10; C12N9/50; C12N9/64; C12N15/00; C12N15/09; C12N15/67; C12N15/69; C12N15/70; C12R1/19; C07H; C12N; C12P; (IPC1-7): C12N15/67
Domestic Patent References:
| JPS57172000A | 1982-10-22 |
Attorney, Agent or Firm:
Akira Asamura (2 outside)
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