Title:
【発明の名称】固定化酵素反応のための方法および装置
Document Type and Number:
Japanese Patent JPH06507313
Kind Code:
A
Abstract:
Perfusive matrices (10) useful in immobilized enzyme reactors (IMERs) are disclosed. The matrices define first and second interconnected sets of pores and an intraparticle high surface area (18) capable of immobilizing enzymes, in fluid communication with the second set of pores. The first and second sets of pores are embodied, for example, as the interstices (12) among particles and throughpores (14) within the particles. The particles also contain diffusive transport pores (16). The dimensions of the first and second sets of pores are such that, at fluid flow rates and pressure drops practical for immobilized enzyme reactions, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of diffusion of the substrate and product to and from immobilized enzymes within the particles. This approach allows rapid enzyme loading and substrate conversion reactions at optimal catalytic flow rates.
Inventors:
Afeyan, Nowberby.
Application Number:
JP51138892A
Publication Date:
August 25, 1994
Filing Date:
April 09, 1992
Export Citation:
Assignee:
Perceptive Biosystems, Inc.
International Classes:
C12M1/40; C12N11/00; C12N11/08; C12P1/00; (IPC1-7): C12P1/00; C12M1/40; C12N11/00
Attorney, Agent or Firm:
Kiyoshi Fujino