Title:
【発明の名称】特定DNA配列に選択的に結合する蛋白質に対する偽構築体として有用なオリゴデオキシヌクレオチドおよびオリゴヌクレオチド
Document Type and Number:
Japanese Patent JPH06509704
Kind Code:
A
Abstract:
PCT No. PCT/US92/03205 Sec. 371 Date Sep. 22, 1993 Sec. 102(e) Date Sep. 22, 1993 PCT Filed Apr. 17, 1992Improved DNA structures are disclosed which contain target sequences which bind to control proteins (such as the CREB protein). The structures of the present invention are stable to degradation, and are effective as decoys for control proteins, making it possible to modulate the transcriptional control normally exerted by such control proteins. In addition, there is provided a method to reversibly crosslink oligonucleotides to polypeptides which recognize the oligonucleotide sequence. This method involves synthesizing DNA structures as described above, wherein one or more phosphorothioate diester linkages are incorporated into the resulting oligonucleotide, allowing the phosphorothioate diester-containing oligonucleotide to bind to polypeptides which recognize the sequence of said oligonucleotide, then contacting the polypeptide-bound oligonucleotide with a transition metal reagent.
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Inventors:
Ju, Barbara, Chen, Faye
Oger, Leslie
Oger, Leslie
Application Number:
JP51090192A
Publication Date:
November 02, 1994
Filing Date:
April 17, 1992
Export Citation:
Assignee:
The Saulk Institute For Biological Studies
International Classes:
A61K31/70; A61K31/7135; A61P31/12; A61P35/00; C07H21/00; C07H21/04; C07H23/00; C12N15/00; C12N15/09; (IPC1-7): C12N15/11; A61K31/70; C07H21/04; C07H23/00; C12N15/00
Attorney, Agent or Firm:
Akira Asamura (3 outside)