Title:
【発明の名称】分泌タンパク質をコードする遺伝子の標的への配達
Document Type and Number:
Japanese Patent JPH06510278
Kind Code:
A
Abstract:
Molecular complexes for targeting a gene encoding a secretory protein to a specific cell in vivo and obtaining secretion of the protein by the targeted cell are disclosed. An expressible gene encoding a desired secretory protein is complexed to a conjugate of a cell-specific binding agent and a gene-binding agent. The cell-specific binding agent is specific for a cellular surface structure which mediates internalization of ligands by endocytosis. An example is the asialoglycoprotein receptor of hepatocytes. The gene-binding agent is a compound such as a polycation which stably complexes the gene under extracellular conditions and releases the gene under intracellular conditions so that it can function within a cell. The molecular complex is stable and soluble in physiological fluids and can be used in gene therapy to selectively transfect cells in vivo to provide for production and secretion of a desired secretory protein.
Inventors:
Wu George Wye.
Wilson James M.
Woo Catherine H.
Wilson James M.
Woo Catherine H.
Application Number:
JP50089292A
Publication Date:
November 17, 1994
Filing Date:
June 05, 1992
Export Citation:
Assignee:
UNIVERSITY OF CONNECTICUT
The Board of Regents of the University of Michigan
The Board of Regents of the University of Michigan
International Classes:
A61K47/48; A61K48/00; C12N15/14; C12N15/16; C12N15/87; (IPC1-7): A61K47/48
Attorney, Agent or Firm:
Ishida Kiki